Quantomix Wet EM
Quantomix is a company which makes sealable electron transparent capsules for Wet EM.
I have used these capsules to do some EM on IC21 cells.
I tried uranyl acetate and osmium tetraoxide - standard stains.
I have also tried collodial gold-streptavidin-biotin-phalloidin - to try to label actin specifically.
But most interesting of all - I tried using quantum dots-streptavidin-biotin-phalloidin - to label actin. This allows us to do fluorescent microscopy and electron microscopy on the exact same sample, without further modification.
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