Registry/Measurement kit/Notebook/2007-6-29

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Contents

To do

  1. Colony PCR of promoter tester (We got 3 colonies (1AT3))
  2. Re-try colony PCR of RBS tester
  3. Run them on a gel
  4. MfeI/NsiI digest of E0240
  5. PCR cleanup E0240
  6. Ligate E0240 and 3K3, transform

Colony PCR

  • Trying extension time of 1:45, in case 1:30 was too short
  • Made new dNTP dilution (108 H2O + 3 of each NTP)

Analytic Gel

  • Loading bottom row:
  • Lanes 1-5 and 16-20 empty or ladder; lanes 6-12 rbs tester; lanes 13-15 promoter tester.

Digest

  • Can't digest with both enzymes concurrently according to NEB, so cutting with MfeI first
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