Registry/Measurement kit/Notebook/2007-6-29

To do

1. Colony PCR of promoter tester (We got 3 colonies (1AT3))
2. Re-try colony PCR of RBS tester
3. Run them on a gel
4. MfeI/NsiI digest of E0240
5. PCR cleanup E0240
6. Ligate E0240 and 3K3, transform

Colony PCR

• Trying extension time of 1:45, in case 1:30 was too short
• Made new dNTP dilution (108 H2O + 3 of each NTP)

Analytic Gel

• Loading bottom row:
• Lanes 1-5 and 16-20 empty or ladder; lanes 6-12 rbs tester; lanes 13-15 promoter tester.

Digest

• Can't digest with both enzymes concurrently according to NEB, so cutting with MfeI first