- Reviewed sequencing results:
- None of E0240 worked except E0240-EX, E2. Not sure about ES, M2.
- F2620 isn't right, should try re-ligating and transformibg in DB3.1
- 55-SX-B0031 was backwards
- 57 ES r0040 #1 is good.
- 57 EX "J116" (actually 102): #1 has mutated beginning, #2 could be a poor read
- 57 EX R0040: both (1&2) are good.
- Should try more colonies of E0240
- making o/n of B0034 (S/X) I2055. two colonies worked.
- perhaps the S/X construct is too difficult to use?