RobWarden:M2D1
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Module 2, Day 1 For Next Time
Expected Cells
Hemocytometer
Count:
10 | 16 | |||
5 | 15 |
Average = 11.5 -> 115,000 cells/ml
Diluted by 1:100 -> 1,150 cells/ml
Plated 3 ml/well -> 3,450 cells/well
General Formula
[math]\displaystyle{
\mbox{Expected Cells} = \mbox{Number Plated} * \mbox{Plating Efficiency} * 2^{\frac{\mbox{Time Since Plating} - \mbox{Seating Time}}{\mbox{Doubling Time}}}
}[/math]
My plates
- Number plated = 3,450 cells/well
- Plating efficiency = .25
- Time since plating = 48 hr
- Seating time = 24 hr
- Doubling time = 24 hr
- Expected cells = [math]\displaystyle{ 3450\ \frac{cells}{well} * .25 * 2^{\frac{48\ hr - 24\ hr}{24\ hr}} = 1725\ \frac{cells}{well} }[/math]
GFP V68L
Although this mutation doesn't look like much, it is enough to destabilize the fluorophore and make the protein fluoresce yellow instead of green.
Calcium Solution
[math]\displaystyle{ 20 \times 10^{-3}\ \frac{mol}{L} * 110.99\ \frac{g}{mol} * 50 \times 10^{-3}\ L = 0.111\ g }[/math]