RobWarden:M2D1

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Contents

Module 2, Day 1 For Next Time

Expected Cells

Hemocytometer

Count:

10 16
5 15

Average = 11.5 -> 115,000 cells/ml
Diluted by 1:100 -> 1,150 cells/ml
Plated 3 ml/well -> 3,450 cells/well

General Formula


\mbox{Expected Cells} = \mbox{Number Plated} * \mbox{Plating Efficiency} * 2^{\frac{\mbox{Time Since Plating} - \mbox{Seating Time}}{\mbox{Doubling Time}}}

My plates

  • Number plated = 3,450 cells/well
  • Plating efficiency = .25
  • Time since plating = 48 hr
  • Seating time = 24 hr
  • Doubling time = 24 hr
  • Expected cells = 
3450\ \frac{cells}{well} * .25 * 2^{\frac{48\ hr - 24\ hr}{24\ hr}} = 1725\ \frac{cells}{well}

GFP V68L

Image:RobWardenV68L.jpg

Although this mutation doesn't look like much, it is enough to destabilize the fluorophore and make the protein fluoresce yellow instead of green.

Calcium Solution


20 \times 10^{-3}\ \frac{mol}{L} *
110.99\ \frac{g}{mol} *
50 \times 10^{-3}\ L =
0.111\ g

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