SIL071906-Construction of pSB1A2-J23013
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[TraGr]
PCR JL10/JL11 on Rlambda (?bp, EcoRI/SpeI, Neb2)
Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)
Product is pSB1A2-J23013
JL10 Forward EcoRI oligo to bioBrick TraG:
CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc
JL11 Reverse SpeI oligo to bioBrick TraG:
gacttACTAGTgaagcggctcatatcgtgatc
