Sack: BTAA Peptide Click HPLC
Copper-click Alkyne / Azide Condensation Reaction with peptide alkyne, azide fluor545
conditions- use HPLC protocol that worked for last GxTX TAMRA purification
sample prep: take 80ul reaction add 20ul ACN + 0.5% TFA, spin hard inject supernatant rinse syringe with 150 ul 20% ACN
run in order:
control + N3-545
stop, compare chromatograms for overlap
Pra13 + N3-545- collect fractions!
Pra17 + N3-545- collect fractions!
save 1 ml 350 nm fluorescent fractions in labeled 1.5ml tubes and freeze at -80C
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