Sarah Carratt: Week 4
TERMINOLOGY: Define the four terms.
MM MODELS: Plot the three examples.
MM PARAMETERS: Apply the Lineweaver-Burk technique to determine Vmax and K.
Sarah Carratt 21:00, 6 February 2011 (EST)
- The Michaelis-Menten equation is used to mathematically illustrate the biological conversion of a substrate and enzyme to a complex and then to a product.
- The Lineweaver-Burk experiment is an example of the Michaelis-Menten equation in action. The results were obtained by manipulating the original equation (ie: taking the reciprocal) and converting the MM equation into mx+b format.
- A chemostat reaction is a "test" to find the equilibrium point of a system, where the amount of substance consumed equals the amount produced or the amount into the system equals the amount going out.
- Exponential growth looks very similar to ex when graphed (pattern: more product=faster growth).
Applying Michealis-Menten Models
Estimating Michaelis-Menten Parameters
Vmax = 0.031551
K = 27.10932