Schumer lab: NEB Monarch Tissue HMW DNA extraction

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  • The PacBio or Nanopore ONT whole genome sequencing we do in the lab requires ~2-5ug of DNA per sample that should be greater than 60kb.
  • We use the NEB Monarch HMW DNA tissue kit. (NEB T3060)
  • See NED Monarch HMW Extraction_Sep2023 in the Protocols folder in Dropbox or email baczenas@stanford.edu for the protocol and all necessary reagents and consumables.