Schumer lab: Pooling libraries for sequencing

Pooling samples for sequencing

First, double check that you have compatible indices.

If you are pooling samples at equal ratios, use this file to calculate the dilution necessary to convert your sample to 4-6 nM

• Use the concentration you got from the Qubit
• Use the average basepair length from the bioanalyzer or tape station
• As you enter your sample information, the sheet with automatically populate with the dilution volume

If you want different coverage for different libraries, adjust the target concentration matches your coverage goals. For example, if I wanted 3X as much of library 1, I would target a concentration of 6 nM for library 1 and 3 nM for library 2, to generate a combined library of 4.5 nM.