Silver:Restriction digest protocol
- restriction enzyme1 (20 units/µl)
- restriction enzyme2 (20 units/µl)
- 10X restriction buffer
- 10X BSA
- PCR product DNA
- distilled H2O
- BioBrick vector (BBa_V0002 or BBa_V0100)
- CIP (10 units/µl)
- V - Volume of PCR product DNA obtained (µl).
- C - Concentration of BioBrick vector (µg/µl).
- Reaction tubes
- Digestion of PCR product
Use the following table as a checklist for preparing the reaction in reaction tube (1):
PCR product DNA 10X BSA 10X restriction buffer restriction enzyme1 restriction enzyme2 distilled H2O Restriction Digest - PCR product V 3.5 µl 3.5 µl 0.2 µl 0.2 µl Make up the volume to 35 µl