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- 03:53, 1 November 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/31/Further-ly Optimized Bulk Ideas (New page: Try maybe two or three times as many MTs (4.213 uM) and the same concentration of kinesin (1: 12.6 ratio). *Gilbert: **12uM tubulin **0.2 uM Kinesin *Last Assay: **1.51 uM tubulin **.3...) (top)
- 03:53, 1 November 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/31 (top)
- 03:52, 1 November 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/31 (Autocreate 2009/10/31 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 02:19, 27 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/26/New PEM (New page: As per the to each their own policy, I made some new PEM buffer for myself today. I used .0768 g EDTA, 4.837 g PIPES, 200 uL 1M MgCl, 185 uL DI H2O, 25 mL 1M NaOH, and 1 mL 1N HCl. I made ...) (top)
- 02:12, 27 October 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/26 (top)
- 02:11, 27 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/26 (Autocreate 2009/10/26 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 05:28, 22 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/21/Motility Assay Attempt (New page: Today Andy informed me that the motility assays were no longer working, both with the Heavy PEM and the regular PEM. So to try to narrow the problem down, I made 21 new aliquots of antifad...) (top)
- 04:29, 22 October 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/21 (top)
- 04:28, 22 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/21 (Autocreate 2009/10/21 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 21:27, 14 October 2009 (hist) (diff) N Image:Cytophoslineerror.jpg (top)
- 21:27, 14 October 2009 (hist) (diff) N Image:Cytophoserror.jpg (top)
- 21:26, 14 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/14/CytoPhos Precision Measurements (New page: I discovered on Monday that our PhosFree assay was not to distinguish between Pi concentrations less than about 10 uM apart. So today I decided to try the CytoPhos assay, which is reported...) (top)
- 21:19, 14 October 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/14 (top)
- 21:19, 14 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/14 (Autocreate 2009/10/14 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 02:13, 13 October 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/12/Malachite Green Errors
- 02:09, 13 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/12/Malachite Green Errors (New page: The big problem hovering over the bulk assays is why I can't seems to get any decent measurements from the new, good kinesin (they are about the same as the when I was using the Cytoskelet...)
- 02:08, 13 October 2009 (hist) (diff) N Image:Phosfreeerror.jpg (top)
- 02:07, 13 October 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/12 (top)
- 02:06, 13 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/12 (Autocreate 2009/10/12 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 18:15, 12 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/09/Bradford Assay Prep (New page: While the kinesin from Haiqing is great, there is an issue in that we do not have a clear idea of the concentration. She did tell us that the concentration of the total kinesin was about 5...) (top)
- 18:15, 12 October 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/09 (top)
- 18:13, 12 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10 (Autocreate Month 2009/10 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook) (top)
- 18:13, 12 October 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/10/09 (Autocreate 2009/10/09 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 04:50, 4 October 2009 (hist) (diff) User:TheLarry/Notebook/Larrys Notebook/2009/09/30/After Steve1 (top)
- 13:49, 22 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/21/New Kinesin (New page: Processes: Go off of motility assay model So, dilute the borrowed kinesin 1:15 (20 uL kinesin to 300 uL PEM) MTs: 5uL (@5 mg/ml) +195 uL PEM-T (.125 mg/ml) = (1.369*10^15 kDa/mL) = (2....) (top)
- 13:48, 22 September 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/21 (top)
- 13:47, 22 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/21 (Autocreate 2009/09/21 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 19:38, 15 September 2009 (hist) (diff) N Image:Twobuffers.jpg (top)
- 19:37, 15 September 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/14/What's wrong with all these assays?!? (top)
- 23:15, 14 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/11/Optimal Kinesin/Microtubule Fail (New page: The trouble with the kinetics assays is that it is very difficult to estimate the ratio of kinesin and microtubules that should be used to give optimum results. We want the number of kines...)
- 23:14, 14 September 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/11 (top)
- 23:13, 14 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/11 (Autocreate 2009/09/11 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 23:13, 14 September 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/14/What's wrong with all these assays?!?
- 23:11, 14 September 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/14/What's wrong with all these assays?!?
- 23:10, 14 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/14/What's wrong with all these assays?!? (New page: Andy and I have both been suffering from a complete lack of activity. The common factor we seemed to find was the PEM-T we had been using, and potentially the "goodness" of the entire bat...)
- 23:10, 14 September 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/14 (top)
- 23:08, 14 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/14 (Autocreate 2009/09/14 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 02:32, 8 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/04/Standard Curves using the Pedestal (New page: Thus far, All measurements I have made have been made by using the cuvette aspect of the Nanodrop. So I decided that it was about time to give the pedestal a whirl and see how it measured ...)
- 02:31, 8 September 2009 (hist) (diff) N Image:Pedestalcomp.jpg (top)
- 02:30, 8 September 2009 (hist) (diff) N Image:Pedstand.jpg (top)
- 02:29, 8 September 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/04 (top)
- 02:28, 8 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09 (Autocreate Month 2009/09 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook) (top)
- 02:28, 8 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/09/04 (Autocreate 2009/09/04 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 23:48, 7 September 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/08/31/CytoPhos Phosphate Assay (top)
- 23:47, 7 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/08/31/CytoPhos Phosphate Assay (New page: After doing so much collection and analysis with the PhosFree Phosphate assay from Cytoskeleton, I decided it was probably about time to crack open the CytoPhos assay and see how the two c...)
- 23:46, 7 September 2009 (hist) (diff) N Image:Cytophosfit.jpg (top)
- 23:45, 7 September 2009 (hist) (diff) N Image:Cytophoscomp.jpg (top)
- 23:45, 7 September 2009 (hist) (diff) User:Brigette D. Black/Notebook/Brigettes Notebook/2009/08/31 (top)
- 23:43, 7 September 2009 (hist) (diff) N User:Brigette D. Black/Notebook/Brigettes Notebook/2009/08/31 (Autocreate 2009/08/31 Entry for User:Brigette_D._Black/Notebook/Brigettes_Notebook)
- 03:35, 31 August 2009 (hist) (diff) User:Linh N Le/Notebook/2009/08/28 (top)
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