User contributions for Colin P. McNally

12 August 2011

• 17:3517:35, 12 August 2011 diff hist +55‎ McClean:Protocols ‎ →‎Flow Cytometry
• 17:3217:32, 12 August 2011 diff hist +1,206‎ N McClean:Phase Plane Mapping ‎ New page: ==Purpose== Expose a strain of yeast to all combinations of two ranges of stimulants, in this case alpha-factor and sorbitol, and measure their response with fluorescent markers and flow c... current
• 14:1714:17, 12 August 2011 diff hist +58‎ McClean:Protocols ‎ →‎Flow Cytometry
• 14:1214:12, 12 August 2011 diff hist +285‎ McClean:Induction Timecourse ‎No edit summary current
• 13:0113:01, 12 August 2011 diff hist +1,232‎ N McClean:Induction Timecourse ‎ New page: #Fill falcon tubes with 800 microL PBS + .1% tween, put on ice #Grow up 25ml of yMM736 to mid-log phase in a klett flask (aim for klett 50-60). Have one klett flask for each condition to ...
• 12:0412:04, 12 August 2011 diff hist +77‎ McClean:Protocols ‎ →‎Flow Cytometry
• 12:0312:03, 12 August 2011 diff hist +916‎ N McClean:Cycloheximide Concentration ‎ New page: ==Purpose== Find the minimum concentration of cyclohexamide required to fully halt translation in S. cerevisae. ==Protocol== #Fill falcon tubes with 800 microL PBS + .1% tween, put on i... current
• 11:5511:55, 12 August 2011 diff hist +14‎ McClean: Fluorescent Folding Time Assay ‎ →‎Notes current
• 11:5411:54, 12 August 2011 diff hist +439‎ McClean: Fluorescent Folding Time Assay ‎ →‎Protocol
• 11:4811:48, 12 August 2011 diff hist +3‎ McClean: Fluorescent Folding Time Assay ‎ →‎Overview
• 11:3811:38, 12 August 2011 diff hist +502‎ McClean: Fluorescent Folding Time Assay ‎ →‎Overview
• 11:3711:37, 12 August 2011 diff hist +1,241‎ N McClean:Fluorescent Protein Start ‎ New page: ==Purpose== Find the earliest detectable fluorescent protein after induction. ==Protocol== #Fill falcon tubes with 800 microL PBS + .1% tween, put on ice #Grow up 25ml of yMM736 to mid-... current
• 09:4609:46, 12 August 2011 diff hist +4‎ McClean:FISH ‎ →‎Protocol
• 09:3909:39, 12 August 2011 diff hist +5,441‎ McClean:FISH ‎ →‎Protocol

11 August 2011

• 13:2413:24, 11 August 2011 diff hist +24‎ N McClean:FISH ‎ New page: ==Protocol== #Grow cells
• 13:2313:23, 11 August 2011 diff hist +56‎ McClean:Protocols ‎ →‎Yeast
• 13:2213:22, 11 August 2011 diff hist +661‎ McClean: Fluorescent Folding Time Assay ‎No edit summary
• 12:0912:09, 11 August 2011 diff hist +7‎ McClean:General Flow Cytometry Procedure ‎ →‎Procedure

9 August 2011

• 14:1014:10, 9 August 2011 diff hist +91‎ McClean:Protocols ‎ →‎FACS
• 14:0914:09, 9 August 2011 diff hist +392‎ N McClean:General Flow Cytometry Procedure ‎ New page: ==Materials== #PBS + 0.1% Tween 20 #Falcon 2058 6mL tubes #a sonicator #BD LSRII ==Procedure== #For each sample fill a falcon tube with 0.8mL of PBS + 0.1% Tween and put it on ice #Grow y...
• 13:5013:50, 9 August 2011 diff hist +14‎ McClean: Fluorescent Folding Time Assay ‎No edit summary
• 12:5012:50, 9 August 2011 diff hist +85‎ McClean:Protocols ‎ →‎FACS
• 12:4812:48, 9 August 2011 diff hist +55‎ Nm McClean: Fluorescent Folding Time Assay ‎ New page: ==Materials== #yeast #liquid media #tubes #FACS machine
• 12:4212:42, 9 August 2011 diff hist +10‎ McClean:Protocols ‎No edit summary
• 12:3512:35, 9 August 2011 diff hist −29‎ User:Colin P. McNally ‎ →‎Research interests current
• 12:3412:34, 9 August 2011 diff hist −5‎ User:Colin P. McNally ‎ →‎Contact Info

27 July 2011

• 10:5610:56, 27 July 2011 diff hist −45‎ User:Colin P. McNally ‎ →‎Education
• 10:5110:51, 27 July 2011 diff hist +3,212‎ N User talk:Colin P. McNally ‎No edit summary current
• 10:5110:51, 27 July 2011 diff hist +1,190‎ N User:Colin P. McNally ‎No edit summary