User contributions for Eli Papa
From OpenWetWare
Jump to navigationJump to search
18 April 2008
- 06:4606:46, 18 April 2008 diff hist −122 Love:Extract unreacted monomer No edit summary current
17 April 2008
- 21:2121:21, 17 April 2008 diff hist −6 Love:TCMedia:Hybridoma No edit summary current
- 12:0712:07, 17 April 2008 diff hist +10 Love:μEn hybridoma screening No edit summary current
- 11:5311:53, 17 April 2008 diff hist −135 Love:μEn hybridoma screening No edit summary
6 February 2008
- 17:0917:09, 6 February 2008 diff hist +57 Love:elisa No edit summary current
- 17:0917:09, 6 February 2008 diff hist +21 Love:elisa No edit summary
- 16:3516:35, 6 February 2008 diff hist +724 Love:elisa No edit summary
- 16:2516:25, 6 February 2008 diff hist +1,069 N Love:elisa New page: == Test Bleed == # Anesthesize animal # Incision on the tail vein # collect necessary amount == Serum Prep == #Let collected blood clot for 30-60min at 37degC, RT for 15min # (optional) S...
- 15:2615:26, 6 February 2008 diff hist +36 Love:Protocols →Immunization
31 January 2008
- 12:1212:12, 31 January 2008 diff hist +25 Love:Meetings No edit summary current
- 10:1110:11, 31 January 2008 diff hist +114 Love:Meetings No edit summary
24 January 2008
- 15:3915:39, 24 January 2008 diff hist +7 Love:injections No edit summary current
- 15:3715:37, 24 January 2008 diff hist +161 Love:injections No edit summary
- 09:1409:14, 24 January 2008 diff hist +15 Love:Equipment No edit summary current
- 09:0509:05, 24 January 2008 diff hist +13 Love:Meetings No edit summary
- 09:0009:00, 24 January 2008 diff hist +80 N Love:Meetings New page: ==Meetings with Chris== ==Group Meetings== * IAP: tuesdays & thursdays at 10am
- 08:5808:58, 24 January 2008 diff hist +129 Template:Love No edit summary current
- 08:5408:54, 24 January 2008 diff hist +167 Love:Equipment No edit summary
- 08:4008:40, 24 January 2008 diff hist +146 Love:Equipment No edit summary
16 January 2008
- 10:2410:24, 16 January 2008 diff hist +138 Love:A20bcells No edit summary current
- 10:1810:18, 16 January 2008 diff hist +560 Love:Medias No edit summary current
- 10:1010:10, 16 January 2008 diff hist +215 N Love:A20bcells New page: * media: supplemented RPMI * usually passed every other day (1:20) * cells/ml are difficult to keep, as these cells grow FAST; usually eyeballed between 10^5 and 10^7, as l...
- 09:4809:48, 16 January 2008 diff hist +58 Love:Protocols No edit summary
14 January 2008
- 16:2116:21, 14 January 2008 diff hist +57 Love:injections No edit summary
- 16:2016:20, 14 January 2008 diff hist +1,707 N Love:injections New page: ==Prepare immunogen== * 50 ug of antigen * 200ul of final injection volume per mice ** 100ul of antigen + PBS ** 100ul of adjuvant * allow some extra room for dead volumes in syringes, e...
- 15:5015:50, 14 January 2008 diff hist +57 Love:Protocols No edit summary
2 December 2007
- 15:3215:32, 2 December 2007 diff hist +1,150 N Love:Plasma cleaning New page: Organic extraction of PDMS SOP #6 Craig Story 1/9/06 A certain amount of monomer/short polymer and catalyst may remain within the cured PDMS. The degree to which these mo... current
- 15:3215:32, 2 December 2007 diff hist +1,150 N Love:Extract unreacted monomer New page: Organic extraction of PDMS SOP #6 Craig Story 1/9/06 A certain amount of monomer/short polymer and catalyst may remain within the cured PDMS. The degree to which these mo...
- 15:3115:31, 2 December 2007 diff hist +4,927 N Love:PDMS pouring New page: PDMS protocol SOP #5 Craig Story 1/9/07 Modified from: http://microsystems.stanford.edu/merl/merl/OperatingProcedures/PDMS.doc Accessed 1/4/07 Safety: Polydimethylsiloxane (PDMS...
- 15:3115:31, 2 December 2007 diff hist +1,599 N Love:klh conjugation New page: KLH conjugation to peptide SOP#2 Written 1/8/07 C. Story The following procedure was used to conjugate 6 peptides with terminal cysteines to KLH. 1. Dissolve 21 mg KLH (...
- 15:3015:30, 2 December 2007 diff hist +3,562 N Love:streptavidin probe New page: Cell Fusion Procedure: SOP #4 by Craig Story Updated 10/18/06, 11/13/06, 1/9/07 Prepare and collect the following needed reagents and supplies day before fusion: *500 ml DME... current
- 15:3015:30, 2 December 2007 diff hist +3,562 N Love:Cell Fusion New page: Cell Fusion Procedure: SOP #4 by Craig Story Updated 10/18/06, 11/13/06, 1/9/07 Prepare and collect the following needed reagents and supplies day before fusion: *500 ml DME...
- 15:3015:30, 2 December 2007 diff hist +2,009 N Love:Medias New page: Media for Hybridoma Fusion, Selection and Maintenance SOP#1 Written 1/8/07 C. Story updated 4/28/07 Formulation of media for Myeloma cells DMEM supplemented with: 10% Fetal B...
- 15:2915:29, 2 December 2007 diff hist +5,460 N Love:μEn hybridoma screening New page: Microengraving Screening for Hybridomas of Interest SOP#8 Craig Story updated 1/9/07 Summary of protocol: 1. Prepare grids (plasma treat, block) 2. Coat epoxy treated s...
- 15:2815:28, 2 December 2007 diff hist +6,080 N Love:Genepix New page: Genepix Capture and Analysis SOP #10 Craig Story 1/9/07, modif 11/2/07 A. Genepix Capture. Tips and things to keep in mind. It is good idea to look over the manual from Axon...
- 15:2815:28, 2 December 2007 diff hist +161 Love:Protocols No edit summary
- 15:2315:23, 2 December 2007 diff hist +311 Love:Protocols No edit summary
- 14:2614:26, 2 December 2007 diff hist 0 Love:SPARC bench No edit summary current
- 14:2014:20, 2 December 2007 diff hist +22 Love:SPARC bench No edit summary
- 14:1914:19, 2 December 2007 diff hist +54 N Love:SPARC bench New page: # Eppendorfs # Tips # Petri dishes # Magnifying Glass
- 14:1214:12, 2 December 2007 diff hist −10 Love:Equipment No edit summary
- 14:1014:10, 2 December 2007 diff hist +49 N Love:Equipment New page: ==Shopping Lists== Love:Equipment:SPARC bench
9 November 2007
- 10:2510:25, 9 November 2007 diff hist +5,295 N Love:Protocols:Counting New page: Cell Counting (in the metamorph era) [Cut] Staining Scan slide 1.place slide clean a glass slide thoroughly with ethanol; let dry and then carefully place the grid on the slid... current
- 10:2510:25, 9 November 2007 diff hist +29 Love:Protocols →μEn
- 08:5808:58, 9 November 2007 diff hist +45 N Love:Genepix analysis Love:Genepix analysis moved to Love:Protocols:Genepix analysis current
- 08:5808:58, 9 November 2007 diff hist 0 m Love:Protocols:Genepix analysis Love:Genepix analysis moved to Love:Protocols:Genepix analysis current
- 08:5708:57, 9 November 2007 diff hist +35 N Genepix analysis Genepix analysis moved to Love:Genepix analysis
- 08:5708:57, 9 November 2007 diff hist 0 m Love:Protocols:Genepix analysis Genepix analysis moved to Love:Genepix analysis
- 08:5508:55, 9 November 2007 diff hist +1,803 N Love:Protocols:Genepix analysis New page: Genepix Analysis 1. open slide as obtained from the scanner 2. rotate the slide recall genepix reads from top to bottom, left to right. block 20 should be on top 3. save rot...
- 08:5108:51, 9 November 2007 diff hist +49 N Love:Protocols New page: ==μEn== ==μChannels for live cell imaging==
