User contributions for Joseph W. Foley

15 June 2023

• 02:4802:48, 15 June 2023 diff hist −40 SPRI bead mix →Other formats: remove DOCX as no longer supported by wiki software current
• 02:4702:47, 15 June 2023 diff hist 0 File:SPRI buffers v2 3.pdf Joseph W. Foley uploaded a new version of File:SPRI buffers v2 3.pdf current

26 June 2018

• 00:3800:38, 26 June 2018 diff hist −6 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific) current
• 00:3800:38, 26 June 2018 diff hist +5 Quantifying sequence libraries with qPCR →Diluting sample libraries
• 00:3700:37, 26 June 2018 diff hist −37 Quantifying sequence libraries with qPCR →Example reaction mix (per plate well)
• 00:3700:37, 26 June 2018 diff hist +22 Quantifying sequence libraries with qPCR →Diluting sample libraries
• 00:3600:36, 26 June 2018 diff hist +16 Quantifying sequence libraries with qPCR →Preparing standard dilutions: accuracy suggestions
• 00:2900:29, 26 June 2018 diff hist +41 Quantifying sequence libraries with qPCR →Preparing standard dilutions
• 00:2900:29, 26 June 2018 diff hist −82 Quantifying sequence libraries with qPCR →Preparing oligonucleotide stocks: no foil
• 00:2800:28, 26 June 2018 diff hist +68 Quantifying sequence libraries with qPCR →Preparing oligonucleotide stocks: suggest premix
• 00:2700:27, 26 June 2018 diff hist +123 Quantifying sequence libraries with qPCR add UDG treatment
• 00:2400:24, 26 June 2018 diff hist +98 Quantifying sequence libraries with qPCR →Preparing standard dilutions: make single-use aliquots
• 00:2300:23, 26 June 2018 diff hist +172 Quantifying sequence libraries with qPCR change purchasing recommendation to dark quencher
• 00:1100:11, 26 June 2018 diff hist +9 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): change fluorophore and quencher to generic labels

3 June 2016

• 14:4514:45, 3 June 2016 diff hist +6 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): bold link

2 June 2016

• 19:5219:52, 2 June 2016 diff hist −6 User:Joseph W. Foley →Contact Info current
• 17:5417:54, 2 June 2016 diff hist +6 m Pooling libraries for sequencing →Pooling criteria: bold worksheet link current
• 17:4817:48, 2 June 2016 diff hist +166 Quantifying sequence libraries with qPCR →Diluting sample libraries: suggestion about plate, pipet, plastics
• 17:4617:46, 2 June 2016 diff hist +60 Quantifying sequence libraries with qPCR mix very well
• 17:4017:40, 2 June 2016 diff hist −87 Quantifying sequence libraries with qPCR Undo revision 942268 by Joseph W. Foley (Talk) - actually it may depend on the specific manufacturer's setup
• 17:3917:39, 2 June 2016 diff hist +87 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): added nmol/OD260
• 17:3517:35, 2 June 2016 diff hist +251 m Pooling libraries for sequencing →Why your results still won't be perfect
• 17:2717:27, 2 June 2016 diff hist 0 m Pooling libraries for sequencing moved elements to right places
• 17:2617:26, 2 June 2016 diff hist +761 Pooling libraries for sequencing why pooling isn't perfect
• 17:1817:18, 2 June 2016 diff hist +296 Pooling libraries for sequencing →Library preparation: added note about color balance

1 June 2016

• 22:5922:59, 1 June 2016 diff hist +72 User:Joseph W. Foley →Contact Info
• 22:5822:58, 1 June 2016 diff hist +11 User:Joseph W. Foley No edit summary
• 21:2821:28, 1 June 2016 diff hist +149 User:Joseph W. Foley →Curated pages

11 May 2016

• 22:1322:13, 11 May 2016 diff hist 0 File:Library pooling worksheet.ods uploaded a new version of "Image:Library pooling worksheet.ods": made example numbers less ambiguous current
• 12:0312:03, 11 May 2016 diff hist +242 Pooling libraries for sequencing added math example just in case
• 11:5811:58, 11 May 2016 diff hist 0 m Pooling libraries for sequencing ordered lists to unordered
• 11:5511:55, 11 May 2016 diff hist +4,515 N Pooling libraries for sequencing page created
• 11:5111:51, 11 May 2016 diff hist +77 File:Library pooling worksheet.ods No edit summary
• 11:5011:50, 11 May 2016 diff hist 0 N File:Library pooling worksheet.ods No edit summary

9 May 2016

• 17:1117:11, 9 May 2016 diff hist +2 m Quantifying sequence libraries with qPCR →Preparing standard dilutions: consistent punctuation
• 17:1017:10, 9 May 2016 diff hist +7 m File:Library qPCR example std-crv1.jpg fixed text and added link current
• 17:0917:09, 9 May 2016 diff hist +12 m File:Library qPCR example amp-plot1.jpg fixed text and added link current
• 17:0817:08, 9 May 2016 diff hist 0 m SPRI bead mix →Other formats: fixed file links
• 17:0517:05, 9 May 2016 diff hist 0 m Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): fixed file link
• 16:5716:57, 9 May 2016 diff hist +74 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): added IDT purchasing info
• 16:5116:51, 9 May 2016 diff hist +329 N File:Illumina qPCR oligos for IDT.txt Primer and probe designs for Illumina library qPCR, formatted for IDT purchasing (use the "Bulk Entry" button on the [http://www.idtdna.com/site/order/oligoentry "Custom DNA" interface]). Note that IDT's "/36-TAMSp/" modification includes TAMRA conjugated current
• 16:4916:49, 9 May 2016 diff hist +223 N File:Illumina qPCR oligos for IDT.tsv Primer and probe designs for Illumina library qPCR, formatted for IDT purchasing. Note that IDT's "/36-TAMSp/" modification includes TAMRA conjugated to a dT, so the final T is omitted from the sequence of unmodified bases. current
• 06:1606:16, 9 May 2016 diff hist +40 Quantifying sequence libraries with qPCR added categories
• 06:1306:13, 9 May 2016 diff hist 0 File:Critical step.png uploaded a new version of "Image:Critical step.png": fixed typo current
• 06:1006:10, 9 May 2016 diff hist +18 m Quantifying sequence libraries with qPCR linked qPCR page
• 06:0506:05, 9 May 2016 diff hist +38 File:Library qPCR example std-crv1.jpg No edit summary
• 06:0506:05, 9 May 2016 diff hist +38 File:Library qPCR example amp-plot1.jpg No edit summary
• 06:0406:04, 9 May 2016 diff hist 0 File:Library qPCR example std-crv1.jpg uploaded a new version of "Image:Library qPCR example std-crv1.jpg"
• 06:0206:02, 9 May 2016 diff hist +490 Quantifying sequence libraries with qPCR added example results
• 05:5705:57, 9 May 2016 diff hist +298 File:Library qPCR example std-crv1.jpg No edit summary