User contributions for Sadao Ota
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20 May 2009
- 12:4412:44, 20 May 2009 diff hist +5 SBB09Ntbk-Sadao Ota →Take out the supernatents, getting rid of background of cells current
- 12:4312:43, 20 May 2009 diff hist 0 SBB09Ntbk-Sadao Ota No edit summary
- 12:4312:43, 20 May 2009 diff hist +1 SBB09Ntbk-Sadao Ota →take out the supernatents, getting rid of background of cells
- 12:4112:41, 20 May 2009 diff hist +7 SBB09Ntbk-Sadao Ota No edit summary
- 12:4012:40, 20 May 2009 diff hist +153 SBB09Ntbk-Sadao Ota →Wednesday:
- 12:3712:37, 20 May 2009 diff hist +203 SBB09Ntbk-Sadao Ota →Sadao 14:02, 6 May 2009 (EDT)
- 12:3312:33, 20 May 2009 diff hist −34 SBB09Ntbk-Sadao Ota →Wednesday:
- 12:3012:30, 20 May 2009 diff hist +2,126 SBB09Ntbk-Sadao Ota No edit summary
- 12:2512:25, 20 May 2009 diff hist −25 SBB09Ntbk-Sadao Ota →Results from incubation on Monday
- 12:2412:24, 20 May 2009 diff hist +7 SBB09Ntbk-Sadao Ota →Results from incubation on Monday
- 12:2312:23, 20 May 2009 diff hist +535 SBB09Ntbk-Sadao Ota →Sadao 14:02, 29 April 2009 (EDT)
- 12:2112:21, 20 May 2009 diff hist +333 SBB09Ntbk-Sadao Ota No edit summary
- 12:2012:20, 20 May 2009 diff hist +964 SBB09Ntbk-Sadao Ota No edit summary
- 12:1512:15, 20 May 2009 diff hist +10 SBB09Ntbk-Sadao Ota →Sadao 14:02, 27 April 2009 (EDT)
- 12:1112:11, 20 May 2009 diff hist +110 SBB09Ntbk-Sadao Ota No edit summary
15 May 2009
29 April 2009
- 03:1503:15, 29 April 2009 diff hist −10 SBB09Ntbk-Sadao Ota No edit summary
- 03:1403:14, 29 April 2009 diff hist −220 SBB09Ntbk-Sadao Ota No edit summary
- 03:1403:14, 29 April 2009 diff hist +2,260 SBB09Ntbk-Sadao Ota No edit summary
- 03:0503:05, 29 April 2009 diff hist −10 SBB09Ntbk-Sadao Ota →Sadao 13:20, 22 April 2009 (EDT)
22 April 2009
- 12:2612:26, 22 April 2009 diff hist +882 SBB09Ntbk-Sadao Ota No edit summary
- 12:2012:20, 22 April 2009 diff hist −3 SBB09Ntbk-Sadao Ota →Sadao 13:41, 20 April 2009 (EDT)
- 12:1912:19, 22 April 2009 diff hist +38 SBB09Ntbk-Sadao Ota →Preparation of Tris buffer solution
- 12:1312:13, 22 April 2009 diff hist +2,448 SBB09Ntbk-Sadao Ota No edit summary
9 March 2009
- 12:3712:37, 9 March 2009 diff hist +98 SBB09 seq No edit summary
- 11:4611:46, 9 March 2009 diff hist +46 SBB09 seq No edit summary
6 March 2009
- 14:1414:14, 6 March 2009 diff hist +22 SBB09 seq No edit summary
4 March 2009
- 13:1113:11, 4 March 2009 diff hist −2 SBB09 seq No edit summary
- 13:1013:10, 4 March 2009 diff hist +7 SBB09 seq No edit summary
25 February 2009
- 13:1113:11, 25 February 2009 diff hist +354 N SBB09 Ligation of EcoRI/BamHI digests New page: *Set up the following reaction: 6.5uL ddH2O 1uL T4 DNA Ligase Buffer (small red or black-striped tubes) 1uL Vector digest 1uL Insert digest 0.5uL T4 DNA Ligase *Pound upside down... current
- 13:1113:11, 25 February 2009 diff hist −310 SBB09Ntbk-Sadao Ota No edit summary
- 13:0913:09, 25 February 2009 diff hist +4 SBB09Ntbk-Sadao Ota →Sadao Ota 15:03, 23 February 2009 (EST)
- 13:0813:08, 25 February 2009 diff hist −406 SBB09Ntbk-Sadao Ota No edit summary
- 13:0713:07, 25 February 2009 diff hist +405 N SBB09 PCR Cloning New page: '''Dilute oligonucleotides to be 10 uM''' * Add 1 ul of oligo dilution to 9 ul of ddH20 * Mix and spin '''Cloning PCR, (products are 937bp & 1600bp, )''' * 24 uL water * 3.3 uL Expand 10... current
- 13:0713:07, 25 February 2009 diff hist +43 SBB09Ntbk-Sadao Ota No edit summary
- 13:0413:04, 25 February 2009 diff hist −52 SBB09Ntbk-Sadao Ota No edit summary
- 13:0413:04, 25 February 2009 diff hist +357 N SBB-Protocols Digest New page: *Set up the following reaction: 8uL of eluted PCR product 1uL of NEB Buffer 2 0.5uL EcoRI 0.5uL BamHI *Incubate at 37 degrees on the thermocycler for 1hr *Clean Up again as describ... current
- 13:0413:04, 25 February 2009 diff hist −327 SBB09Ntbk-Sadao Ota No edit summary
- 13:0213:02, 25 February 2009 diff hist +49 N SBB-Protocols Gelation Ana New page: mix the eluted DNAs (3 ul) and loading dye (2 ul) current
- 13:0213:02, 25 February 2009 diff hist +37 SBB09Ntbk-Sadao Ota No edit summary
- 12:5912:59, 25 February 2009 diff hist +478 N SBB-Protocols Zymo1 New page: #Add 180 uL of Zymo ADB buffer (brown bottle) to a 33uL or 50uL reaction. #Transfer into the Zymo column (small clear guys) #spin through, discard waste. #Add 200 uL of PE or Zymo Wash buf... current
- 12:5912:59, 25 February 2009 diff hist −478 SBB09Ntbk-Sadao Ota No edit summary
- 12:5812:58, 25 February 2009 diff hist +179 SBB09Ntbk-Sadao Ota No edit summary
- 11:4111:41, 25 February 2009 diff hist +458 SBB09Ntbk-Sadao Ota No edit summary
23 February 2009
- 23:2223:22, 23 February 2009 diff hist −71 SBB09Ntbk-Sadao Ota No edit summary
- 23:2123:21, 23 February 2009 diff hist +157 SBB09Ntbk-Sadao Ota →Sadao Ota 15:03, 23 February 2009 (EST)
- 23:2023:20, 23 February 2009 diff hist −320 SBB09Ntbk-Sadao Ota No edit summary
- 23:1823:18, 23 February 2009 diff hist +511 SBB09Ntbk-Sadao Ota No edit summary
- 23:1323:13, 23 February 2009 diff hist +3 SBB09Ntbk-Sadao Ota No edit summary
- 23:1123:11, 23 February 2009 diff hist +1,114 SBB09Ntbk-Sadao Ota No edit summary
