User contributions for Sarah Zarrin

8 February 2009

• 21:0521:05, 8 February 2009 diff hist −598‎ Jacobs:Back Door ‎ →‎Research Protocols
• 21:0421:04, 8 February 2009 diff hist +604‎ Jacobs:Back Door ‎ →‎Teaching Protocols

7 January 2009

• 21:5121:51, 7 January 2009 diff hist +83‎ Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins ‎ →‎Procedure

22 July 2008

• 06:1306:13, 22 July 2008 diff hist +87‎ Jacobs:Protocol Freezing and Thawing Cells ‎No edit summary

17 July 2008

• 08:2008:20, 17 July 2008 diff hist +136‎ Jacobs:Back Door ‎No edit summary
• 08:0208:02, 17 July 2008 diff hist 0‎ Jacobs:Lab Members ‎ →‎Current (Stanford)
• 07:4707:47, 17 July 2008 diff hist +33‎ Jacobs:Lab Members ‎ →‎Current (Stanford)
• 07:4207:42, 17 July 2008 diff hist −18‎ Jacobs:Lab Members ‎ →‎Current (Stanford)
• 07:3907:39, 17 July 2008 diff hist −12‎ Jacobs:Lab Members ‎ →‎Current (Stanford)
• 07:3007:30, 17 July 2008 diff hist +112‎ Jacobs:Protocol Freezing and Thawing Cells ‎ →‎Procedure
• 07:2707:27, 17 July 2008 diff hist +2‎ Jacobs:Protocol Freezing and Thawing Cells ‎ →‎Procedure
• 07:2607:26, 17 July 2008 diff hist +100‎ Jacobs:Protocol Freezing and Thawing Cells ‎ →‎Procedure
• 07:2107:21, 17 July 2008 diff hist +48‎ Jacobs:Protocol Freezing and Thawing Cells ‎ →‎Procedure

15 June 2008

• 20:5220:52, 15 June 2008 diff hist +2,658‎ N Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin ‎ New page: ==Materials== * 3T3 Fibroblasts (seeded in 35 mm dishes or a 6-well plate) * FuGENE 6 transfection reagent (Roche Applied Science Cat# 11 815 091 001) * pAcGFP1-Actin vector (Clontech Cat... current
• 20:4420:44, 15 June 2008 diff hist +95‎ Jacobs:Back Door ‎ →‎Research Protocols
• 20:4220:42, 15 June 2008 diff hist +2,720‎ N Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer ‎ New page: ==Materials== * RIPA buffer (RIPA buffer enables the extraction of cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein a... current
• 20:3420:34, 15 June 2008 diff hist +117‎ Jacobs:Back Door ‎ →‎Research Protocols
• 20:3120:31, 15 June 2008 diff hist +4,468‎ N Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis ‎ New page: ==Materials== * Agarose * Formaldehyde * 10X MOPS buffer * RNAse free water * Ethidium Bromide * Glass bottle * RNA Sample * Microwave * Agarose gel electrophoresis system (gel box, castin... current
• 20:2220:22, 15 June 2008 diff hist +143‎ Jacobs:Back Door ‎ →‎Research Protocols
• 15:3715:37, 15 June 2008 diff hist +4,193‎ N Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein ‎ New page: ==Overview== Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method used to separate proteins based on their size. In their native state, proteins fold into uniq...
• 15:3015:30, 15 June 2008 diff hist +288‎ Jacobs:Back Door ‎ →‎Research Protocols
• 15:2515:25, 15 June 2008 diff hist +1,819‎ N Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain ‎ New page: ==Materials== * Polyacrylamide pre-cast gel * SimplyBlue™ Coomassie protein stain, 1x pre-mixed solution (Coomassie blue dye binds to proteins (arginine, the aromatic amino acids, and hi... current
• 15:2015:20, 15 June 2008 diff hist +133‎ Jacobs:Back Door ‎ →‎Research Protocols
• 15:1815:18, 15 June 2008 diff hist +1,491‎ N Jacobs:Protocol Pipetting Exercise ‎ New page: ==Materials== * Pipetman (20ul, 100ul, 1000ul) * Pipet tips * 1.5 ml microcentrifuge tubes * Beaker with distilled water * Analytical balance * Laboratory marking pen ==Procedure== # Labe... current
• 15:1315:13, 15 June 2008 diff hist +60‎ Jacobs:Back Door ‎ →‎Teaching Protocols
• 15:0915:09, 15 June 2008 diff hist +3,675‎ N Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel ‎ New page: ==Overview== PCR is used to amplify specific regions of DNA. It can amplify a single gene, parts of a gene or non-coding regions. We will use PCR to amplify the FAK and Cre sequences in m... current
• 14:4714:47, 15 June 2008 diff hist +139‎ Jacobs:Back Door ‎ →‎Research Protocols
• 14:4414:44, 15 June 2008 diff hist +3,238‎ N Jacobs:Protocol Actin Staining ‎ New page: ==Materials== *Cells: NIH/3T3 fibroblasts seeded @1,000 to 3,000 cells/cm2 24 to 48 hrs prior to staining *Coverslips *Kimwipes *Aluminum Foil *Pipets/Pipet aid *Pipet tips/pipetters *Asp... current
• 14:0514:05, 15 June 2008 diff hist +51‎ Jacobs:Back Door ‎ →‎Research Protocols

13 June 2008

• 14:5514:55, 13 June 2008 diff hist +3,192‎ N User talk:Sarah Zarrin ‎No edit summary current
• 14:5514:55, 13 June 2008 diff hist +1,136‎ N User:Sarah Zarrin ‎No edit summary current