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bibtexCitation bibtexEntryType author title journal year month day volume issue pages keywords note issn url abstract
Benner2005aarticleBenner, Steven A. ; Sismour, A. MichaelSynthetic biologyNature reviews. Genetics.2005Julxx67533--543synthetic biology10.1038/nrg16371471-0056http://www.nature.com/nrg/journal/v6/n7/abs/nrg1637.html
Brenner2008articleBrenner, Katie ; You, Lingchong ; Arnold, Frances H.Engineering microbial consortia: a new frontier in synthetic biology.Trends in biotechnology2008Jul31biotechnology10.1016/j.tibtech.2008.05.0040167-7799http://www.ncbi.nlm.nih.gov/pubmed/18675483
Kodumal2004articleKodumal, Sarah ; Patel, Kedar ; Reid, Ralph ; Menzella, Hugo ; Welch, Mark ; Santi, DanielTotal synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene clusterProceedings of the National Academy of Sciences of the United States of America2004Nov021014415573--15578synthetic biology10.1073/pnas.0406911101http://www.pnas.org/content/101/44/15573.abstract
Hammann2007articleHammann, Christian ; Westhof, EricSearching genomes for ribozymes and riboswitchesGenome Biology2007Apr3084210synthetic biology10.1186/gb-2007-8-4-210http://genomebiology.com/2007/8/4/210
Arkin2008articleArkin, AdamSetting the standard in synthetic biologyNat Biotech2008Julxx267771--774OPINION10.1038/nbt0708-771http://www.nature.com/nbt/journal/v26/n7/full/nbt0708-771.html
Church2005aarticleChurch, GeorgeLet us go forth and safely multiplyNature2005Nov244387067423synthetic biology10.1038/438423a0028-0836http://dx.doi.org/10.1038/438423a
Check2005aarticleCheck, ErikaSynthetic biology: Designs on lifeNature2005Nov244387067417--418synthetic biology10.1038/438417ahttp://dx.doi.org/10.1038/438417a
Pryciak2008articlePryciak, PeterSYSTEMS BIOLOGY: Customized Signaling CircuitsScience2008Mar1431958691489--1490signalling10.1126/science.1156414http://www.sciencemag.org/cgi/content/full/319/5869/1489?ck=nck
ref9articleRewiring Bacteria, Two Components at a TimeCell2008xxxx1336947synthetic biology10.1016/j.cell.2008.05.03500928674http://dx.doi.org/10.1016/j.cell.2008.05.035
Greber2007articleGreber, David ; Fussenegger, MartinMammalian synthetic biology: Engineering of sophisticated gene networksJournal of Biotechnology2007Julxx130329--345Biopharmaceutical manufacturing10.1016/j.jbiotec.2007.05.014http://www.sciencedirect.com/science/article/B6T3C-4NT9G60-6/1/be91f43dd87b2e4e80afa221d5ffbc18With the recent development of a wide range of inducible mammalian transgene control systems it has now become possible to create functional synthetic gene networks by linking and connecting systems into various configurations. The past 5 years has thus seen the design and construction of the first synthetic mammalian gene regulatory networks. These networks have built upon pioneering advances in prokaryotic synthetic networks and possess an impressive range of functionalities that will some day enable the engineering of sophisticated inter- and intra-cellular functions to become a reality. At a relatively simple level, the modular linking of transcriptional components has enabled the creation of genetic networks that are strongly analogous to the architectural design and functionality of electronic circuits. Thus, by combining components in different serial or parallel configurations it is possible to produce networks that follow strict logic in integrating multiple independent signals (logic gates and transcriptional cascades) or which temporally modify input signals (time-delay circuits). Progressing in terms of sophistication, synthetic transcriptional networks have also been constructed which emulate naturally occurring genetic properties, such as bistability or dynamic instability. Toggle switches which possess ``memory'' so as to remember transient administered inputs, hysteric switches which are resistant to stochastic fluctuations in inputs, and oscillatory networks which produce regularly timed expression outputs, are all examples of networks that have been constructed using such properties. Initial steps have also been made in designing the above networks to respond not only to exogenous signals, but also endogenous signals that may be associated with aberrant cellular function or physiology thereby providing a means for tightly controlled gene therapy applications. Moving beyond pure transcriptional control, synthetic networks have also been created which utilize phenomena, such as post-transcriptional silencing, translational control, or inter-cellular signaling to produce novel network-based control both within and between cells. It is envisaged in the not-too-distant future that these networks will provide the basis for highly sophisticated genetic manipulations in biopharmaceutical manufacturing, gene therapy and tissue engineering applications.
ref11electronicBacteria can be turned into living computers - Telegraph - on article in J Biol Engsynthetic biologyhttp://www.telegraph.co.uk/earth/main.jhtml?view=DETAILS&grid=&xml=/earth/2008/05/20/scibacteria120.xmlBacteria have been turned into ``living computers'' and used to solve a mathematical problem. The feat by an American team shows that computing using living cells is feasible, opening the door to a number of applications including data storage. The research is part of a general effort in ``synthetic biology'' to improve on four billion years of evolution and turn bacteria into living machines to do our bidding. The results, published in the Journal of Biological Engineering, are reported by Karmella Haynes and colleagues from the biology and the mathematics departments of Davidson College, North Carolina and Missouri Western State University, Missouri. They added genes to an innocuous form of the gut bacterium Escherichia coli, a workhorse of genetics research, creating bacterial computers able to solve a classic mathematical puzzle, known as the Burnt Pancake Problem.
ref12electronicThe crucial role of CS in systems and synthetic biologycomputer sciencehttp://portal.acm.org/citation.cfm?id=1342332
Bashor2008aarticleBashor, Caleb ; Helman, Noah ; Yan, Shude ; Lim, WendellUsing Engineered Scaffold Interactions to Reshape MAP Kinase Pathway Signaling DynamicsScience2008Mar1431958691539--1543Biotech10.1126/science.1151153http://www.sciencemag.org/cgi/content/abstract/319/5869/1539
Linshiz2008articleLinshiz, Gregory ; ben Yehezkel, Tuval ; Kaplan, Shai ; Gronau, Ilan ; Ravid, Sivan ; Adar, Rivka ; Shapiro, EhudRecursive construction of perfect DNA molecules from imperfect oligonucleotidesMol Syst Biol2008May064DNA synthesis10.1038/msb.2008.26http://www.nature.com/msb/journal/v4/n1/full/msb200826.html
ref15electronicMain Page - 2008.igem.orggenomehttp://2008.igem.org/Main_PageiGEM is the premiere undergraduate Synthetic Biology competition. Student teams are given a kit of biological parts at the beginning of the summer. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells. During the weekend of November 8-9, they present their work at the iGEM Competition Jamboree at MIT and have a chance to win awards. They add their new parts to the Registry of Standard Biological Parts for the students in the next year's competition.
ref16electronicPLoS Computational Biology: Biosynthetic Potentials of Metabolites and Their Hierarchical Organizationto readhttp://www.ploscompbiol.org/article/info%3Adoi%2F10.1371%2Fjournal.pcbi.1000049
ref17electronicPLoS Computational Biology: The Evolution of Robust Development and Homeostasis in Artificial Organismssynthetic biologyhttp://www.ploscompbiol.org/article/info%3Adoi%2F10.1371%2Fjournal.pcbi.1000030
Serrano2007articleSerrano, LuisSynthetic biology: promises and challengesMol Syst Biol2007Dec183synthetic biology10.1038/msb4100202http://www.nature.com/msb/journal/v3/n1/full/msb4100202.html
Doktycz2007articleDoktycz, Mitchel ; Simpson, MichaelNano-enabled synthetic biologyMol Syst Biol2007Jul103synthetic biology10.1038/msb4100165http://www.nature.com/msb/journal/v3/n1/full/msb4100165.html
Bashor2008barticleBashor, Caleb ; Helman, Noah ; Yan, Shude ; Lim, WendellUsing Engineered Scaffold Interactions to Reshape MAP Kinase Pathway Signaling DynamicsScience2008Mar1431958691539--1543synthetic biology10.1126/science.1151153http://www.sciencemag.org/cgi/content/full/319/5869/1539
Gibson2008articleGibson, Daniel G. ; Benders, Gwynedd A. ; Andrews-Pfannkoch, Cynthia ; Denisova, Evgeniya A. ; Baden-Tillson, Holly ; Zaveri, Jayshree ; Stockwell, Timothy B. ; Brownley, Anushka ; Thomas, David W. ; Algire, Mikkel A. ; Merryman, Chuck ; Young, Lei ; Noskov, Vladimir N. ; Glass, John I. ; Venter, J. Craig ; Hutchison, Clyde A. III ; Smith, Hamilton O.Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium GenomeScience (New York, N.Y.)2008Jan2431958671151721--1220Venter10.1126/science.11517211095-9203http://dx.doi.org/10.1126/science.1151721
ref22electronicSynthetic Gene Circuits: Design with Directed Evolution - Annual Review of Biophysics and Biomolecular Structure, 36(1):1 - Abstractreviewhttp://arjournals.annualreviews.org/doi/abs/10.1146/annurev.biophys.36.040306.132600
ref23articleSynthetic Gene Circuits: Design with Directed EvolutionAnnual Review of Biophysics and Biomolecular Structure2007xxxx3611BioBrick Characterisation10.1146/annurev.biophys.36.040306.13260010568700http://dx.doi.org/10.1146/annurev.biophys.36.040306.132600
Sauro2008articleSauro, HerbertModularity definedMol Syst Biol2008Feb124systems biology10.1038/msb.2008.3http://www.nature.com/msb/journal/v4/n1/full/msb20083.htmlcommentary on Del Vecchio et al (2008).
ref25electronicFrom the cells up | Science | The Guardiansynthetic biologyhttp://www.guardian.co.uk/science/2005/mar/10/science.research
ref26electronicLaying the foundations for a bio-economy 2008carlsonhttp://www.springerlink.com/content/n211746672413507/fulltext.pdf
ref27electronichackwriters.com - 21st Century Identity - Memory and Personality - Kim Pfieferemergent evolutionhttp://www.hackwriters.com/identity21.htmThe coming horrors of body-parts manufacture and cloning.
Benders2008articleBenders, Gwynedd ; Hamilton, AndComplete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium GenomeScience2008Jan241151721synthetic biology10.1126/science.1151721http://www.sciencemag.org/cgi/content/abstract/319/5867/1215?sa_campaign=Email/toc/29-February-2008/10.1126/science.1151721We have synthesized a 582,970 bp Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted ``watermarks'' at intergenic sites known to tolerate transposon insertions. Overlapping ``cassettes'' of 5 to 7 kb, assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb (``1/8 genome''), and 144 kb (``1/4 genome''), which were all cloned as bacterial artificial chromosomes (BACs) in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination (TAR) cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments.
ref29electronicGenome stitched together by hand : Nature News - on article in Sciencesynthetic biologyhttp://www.nature.com/news/2008/080124/full/news.2008.522.htmlScientists have succeeded in stitching together an entire bacterial genome, creating in the lab the full set of instructions needed to make a living thing. The stage is now set for the creation of the first artificial organism — and it could be achieved within the year. The genome for the pathogenic bacterium Mycoplasma genitalium was made in the laboratory by Hamilton Smith and his colleagues at the J. Craig Venter Institute in Rockville, Maryland. The genome has 582,970 of the fundamental building blocks of DNA, called nucleotide bases, making it more than a factor of ten longer than the previous-longest stretch of genetic material created by chemical means.
ref30electronicETC Group -synthetic biologyhttp://www.etcgroup.org/en/Synthetic Biology Cartoons
Pennisi2008articlePennisi, ElizabethScientists Synthesize a Genome From Scratch - on article in ScienceScienceNOW2008Jan2420081243synthetic biologyhttp://sciencenow.sciencemag.org/cgi/content/full/2008/124/3Researchers have rebuilt an entire genome from scratch, they report online today in Science. Although the team has yet to demonstrate that this DNA can substitute for the real thing, the work paves the way for customized bacteria that could efficiently produce drugs, biofuels, and other molecules useful to humankind. Ever since his group decoded the genome of Mycoplasma genitalium, a parasitic bacterium that lives in the human urogenital tract, sequencing maverick J. Craig Venter has wanted to remake the bug's genome in the lab. At just under 600,000 bases, M. genitalium sports the smallest known genome for a free-living organism, and Venter hoped that an artificial genome could be modified to turn the bacterium into a living chemical-manufacturing plant.
ref32electronicScientists Take New Step Toward Man-Made Life - on article in Sciencesynthetic biologyhttp://www.nytimes.com/2008/01/24/science/24cnd-genome.htmlTaking a significant step toward the creation of man-made forms of life, researchers reported Thursday that they had manufactured the entire genome of a bacterium by painstakingly stitching together its chemical components. While scientists had previously synthesized the complete DNA of viruses, this is the first time it has been done for bacteria, which are much more complex. The genome is more than 10 times as long as the longest piece of DNA ever previously synthesized.
Sidney2007aarticleSidney, Robert ; Cox, ; Surette, Michael ; Elowitz, MichaelProgramming gene expression with combinatorial promotersMol Syst Biol2007Nov133Promoter10.1038/msb4100187http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html
ref34articleSystems interface biologyJournal of The Royal Society Interface2006xxxx310603stochastic10.1098/rsif.2006.0143http://dx.doi.org/10.1098/rsif.2006.0143
Cox2007articleCox, Robert Sidney ; Surette, Michael ; Elowitz, MichaelProgramming gene expression with combinatorial promotersMol Syst Biol2007Nov133synthetic biology10.1038/msb4100187http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html
ref36articleProgramming and engineering biological networksCurrent Opinion in Structural Biology2006xxxx164551synthetic biology10.1016/j.sbi.2006.06.011http://dx.doi.org/10.1016/j.sbi.2006.06.011
ref37electronicRegistry of Standard Biological Partsregistryhttp://parts.mit.edu/registry/index.php/Main_Page
ref38electronicBioMed Central | Abstract | 1752-0509-1-50 | Noise-induced switches in network systems of the genetic toggle switchnoisehttp://www.biomedcentral.com/1752-0509/1/50
Sidney2007barticleSidney, Robert ; Cox, ; Surette, Michael ; Elowitz, MichaelProgramming gene expression with combinatorial promotersMol Syst Biol2007Nov133synthetic biology10.1038/msb4100187http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html
ref40articleNoise and kinetics of LuxR positive feedback loopsBiochemical and Biophysical Research Communications2007xxxx3633667synthetic biology10.1016/j.bbrc.2007.09.057http://dx.doi.org/10.1016/j.bbrc.2007.09.057
ref41articleDesign and implementation of three incoherent feed-forward motif based biological concentration sensorsSystems and Synthetic Biology2007xxxxsynthetic biology10.1007/s11693-007-9008-618725325http://dx.doi.org/10.1007/s11693-007-9008-6
ref42electronicBBC NEWS | Science/Nature | Creating life in the laboratorysynthetic biologyhttp://news.bbc.co.uk/2/hi/science/nature/7041353.stmThe race to create life version 2.0 is under way. The J Craig Venter Institute scientists are aiming to craft a ``minimal genome''- the smallest group of genes an organism needs to survive and function - and insert it into an empty cell. This stripped-down genome has been established with the help of a simple bacterium, Mycoplasma genitalium, by knocking out its genes, one by one, until only the genetic material vital for survival was found.
ref43articleExpanding the metabolic engineering toolbox: more options to engineer cellsTrends in Biotechnology2007xxxx253132BioBrick Characterisation10.1016/j.tibtech.2007.01.003http://dx.doi.org/10.1016/j.tibtech.2007.01.003
Wu2007articleWu, Gang ; Dress, Laura ; Freeland, StephenOptimal encoding rules for synthetic genes: the need for a community effortMol Syst Biol2007Sep183synthetic biology10.1038/msb4100176http://www.nature.com/msb/journal/v3/n1/full/msb4100176.html
Murphy2007aarticleMurphy, Kevin ; Bal{\'a}zsi, G{\'a}bor ; Collins, JamesCombinatorial promoter design for engineering noisy gene expression.Proceedings of the National Academy of Sciences of the United States of America2007Jul311043112726--12731synthetic biology10.1073/pnas.0608451104http://www.pnas.org/cgi/content/abstract/0608451104v1?maxtoshow=&HITS=1&hits=1&RESULTFORMAT=&andorexacttitle=and&titleabstract=synthetic+biology&andorexacttitleabs=and&andorexactfulltext=and&searchid=1&usestrictdates=yes&resourcetype=HWCIT&ct
Luisi2007articleLuisi, PierChemical Aspects of Synthetic BiologyChemistry \& Biodiversity2007xxxx44603--621synthetic biology10.1002/cbdv.200790053http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17443874&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus
ref47articleSynthetic biology: lessons from the history of synthetic organic chemistryNature Chemical Biology2007xxxx39521--521synthetic biology10.1038/nchembio0907-521http://dx.doi.org/10.1038/nchembio0907-521
ref48electronicPrather Group - Home PageLabhttp://web.mit.edu/prathergroup/index.htmlOur research is centered on the design and assembly of recombinant microorganisms for the production of small molecules. More simply, we are harnessing the synthetic power of biology to build microbial chemical factories. Current efforts include the development of tools and methodologies for novel biosynthetic pathway design and the investigation of gene dosage effects on the physiology and productivity of engineered microbes. Our research combines the traditions of metabolic engineering with the practices of biocatalysis to expand and optimize the biosynthetic capacity of microbial systems. A particular focus is the elucidation of design principles for the production of unnatural organic compounds within the framework of the nascent field of synthetic biology. Major Research Areas
Pedraza2005aarticlePedraza, Juan ; van Oudenaarden, AlexanderNoise propagation in gene networks.Science (New York, N.Y.)2005Mar2530757171965--1969synthetic biology10.1126/science.1109090http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15790857&query_hl=12
Hooshangi2005aarticleHooshangi, Sara ; Thiberge, Stephan ; Weiss, RonUltrasensitivity and noise propagation in a synthetic transcriptional cascadeProceedings of the National Academy of Sciences2005Mar08102103581--3586inverter composition10.1073/pnas.0408507102http://dx.doi.org/10.1073/pnas.0408507102
ref51electronicCell -- Deans et al.synthetic biologyhttp://www.cell.com/content/article/abstract?uid=PIIS0092867407006848
Murphy2007barticleMurphy, Kevin ; Balazsi, Gabor ; Collins, JamesCombinatorial promoter design for engineering noisy gene expressionProceedings of the National Academy of Sciences2007Jul311043112726--12731synthetic biology10.1073/pnas.0608451104http://www.pnas.org/cgi/content/abstract/0608451104v1?maxtoshow=&HITS=1&hits=1&RESULTFORMAT=&andorexacttitle=and&titleabstract=synthetic+biology&andorexacttitleabs=and&andorexactfulltext=and&searchid=1&usestrictdates=yes&resourcetype=HWCIT&ct
Grilly2007articleGrilly, Chris ; Stricker, Jesse ; Pang, Wyming ; Bennett, Matthew ; Hasty, JeffA synthetic gene network for tuning protein degradation in Saccharomyces cerevisiaeMol Syst Biol2007Jul313synthetic biology10.1038/msb4100168http://www.nature.com/msb/journal/v3/n1/full/msb4100168.html
Waldminghaus2007articleWaldminghaus, Torsten ; Gaubig, Lena ; Narberhaus, FranzGenome-wide bioinformatic prediction and experimental evaluation of potential RNA thermometers.Mol Genet Genomics2007Jul24synthetic biology10.1007/s00438-007-0272-7http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17647020&itool=pubmed_DocSum
ref55articleToward an artificial cell based on gene expression in vesicles.Physical Biology2005xxxx231--1synthetic biology10.1088/1478-3975/2/3/p01http://www.ncbi.nlm.nih.gov/pubmed/16224117?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus
Rinaudo2007aarticleRinaudo, Keller ; Bleris, Leonidas ; Maddamsetti, Rohan ; Subramanian, Sairam ; Weiss, Ron ; Benenson, YaakovA universal RNAi-based logic evaluator that operates in mammalian cellsNat Biotech2007Julxx257795--801synthetic biology10.1038/nbt1307http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt1307.html
Szczepek2007articleSzczepek, Michal ; Brondani, Vincent ; B{\"u}chel, Janine ; Serrano, Luis ; Segal, David ; Cathomen, ToniStructure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases.Nature biotechnology2007Julxx257786--793zinc-finger nuclease10.1038/nbt1317http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1317.html
Miller2007articleMiller, Jeffrey ; Holmes, Michael ; Wang, Jianbin ; Guschin, Dmitry ; Lee, Ya-Li ; Rupniewski, Igor ; Beausejour, Christian ; Waite, Adam ; Wang, Nathaniel ; Kim, Kenneth ; Gregory, Philip ; Pabo, Carl ; Rebar, EdwardAn improved zinc-finger nuclease architecture for highly specific genome editing.Nature biotechnology2007Julxx257778--785Protein engineering10.1038/nbt1319http://dx.doi.org/10.1038/nbt1319
Wang2007articleWang, Kaihang ; Neumann, Heinz ; Peak-Chew, Sew ; Chin, JasonEvolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion.Nature biotechnology2007Julxx257770--777orthogonal10.1038/nbt1314http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1314.html
Walter2007articleWalter, Jessica ; Greenfield, Derek ; Bustamante, Carlos ; Liphardt, JanLight-powering Escherichia coli with proteorhodopsinProceedings of the National Academy of Sciences2007Feb1310472408--2412proteorhodopsin10.1073/pnas.0611035104http://dx.doi.org/10.1073/pnas.0611035104
Drubin2007aarticleDrubin, David ; Way, Jeffrey ; Silver, PamelaDesigning biological systems.Genes \& development2007Feb01213242--254be theory10.1101/gad.1507207http://www.genesdev.org/cgi/content/full/21/3/242
Andrianantoandro2006aarticleAndrianantoandro, Ernesto ; Basu, Subhayu ; Karig, David ; Weiss, RonSynthetic biology: new engineering rules for an emerging discipline.Molecular systems biology2006xxxx22006--0028synthetic biology10.1038/msb4100073http://www.nature.com/msb/journal/v2/n1/full/msb4100073.html
Ball2007aarticleBall, PhilipSynthetic biology: Designs for lifeNature2007Jul05448714932--33synthetic biology10.1038/448032ahttp://www.nature.com/nature/journal/v448/n7149/full/448032a.html
Ball2007barticleBall, PhilipSynthetic biology: Designs for life - on article in ScienceNature2007Jul05448714932--33synthetic biology10.1038/448032ahttp://www.nature.com/nature/journal/v448/n7149/full/448032a.htmlThe genome of one bacterium has been successfully replaced with that of a different bacterium, transforming one species into another. This development is a harbinger of whole-genome engineering for practical ends.
Yeh2007aarticleYeh, Brian ; Rutigliano, Robert ; Deb, Anrica ; Bar-Sagi, Dafna ; Lim, WendellRewiring cellular morphology pathways with synthetic guanine nucleotide exchange factorsNature2007May314477144596--600gtpase10.1038/nature05851http://www.nature.com/nature/journal/vaop/ncurrent/abs/nature05851.html
Lartigue2007aarticleLartigue, Carole ; Glass, John ; Alperovich, Nina ; Pieper, Rembert ; Parmar, Prashanth ; Hutchison, III Clyde ; Smith, Hamilton ; Venter, J.Genome Transplantation in Bacteria: Changing One Species to AnotherScience2007Jun281144622--1144622synthetic biology10.1126/science.1144622http://www.sciencemag.org/cgi/content/abstract/1144622My comments: Venter and colleagues describe a method for replacing a chromosome in one bacteria by another from another bacteria (the two bacteria being distinct species, although {\textasciitilde}95\% identical at the genome level in this case). One of the challenges was in the extraction of intact, protein-free chromosomes from cells. The actual transfer then relies on polyethylene glycol (PEG), known to promote fusion of eukaryotic cells, but the actual transplantation mechanism is not understood. The authors hypothesize that two chromosome-free cells might fuse around a naked chromosome, with low frequency (but the resulting cells are selected using an antibiotics resistance gene). Nonetheless, this is clearly an important step forward for synthetic biology (you can imagine that entirely synthesized chromosomes will be inserted into empty host cells using this type of approaches).
ref67electronicScientists Report DNA Transplant - washingtonpost.com - on article in Sciencesynthetic biologyhttp://www.washingtonpost.com/wp-dyn/content/article/2007/06/28/AR2007062802046.htmlScientists said yesterday that they had transplanted a microbe's entire, tangled mass of DNA into a closely related organism, a delicate operation that cleanly transformed the recipient from one species into the other. After the operations, the ``patients'' -- single-celled organisms resembling bacteria -- dutifully obeyed their new genomes and by every measure exhibited the biological personas of the donors.
Pennisi2007articlePennisi, ElizabethGENETICS: Replacement Genome Gives Microbe New IdentityScience2007Jun2931658331827--1827synthetic biology10.1126/science.316.5833.1827ahttp://www.sciencemag.org/cgi/content/full/316/5833/1827aFor decades, molecular biologists have genetically modified microbes and other kinds of cells by adding short DNA sequences, whole genes, and even large pieces of chromosomes. Now, in a feat reported in a paper published online by Science this week (www.sciencemag.org/cgi/content/abstract/1144622), one group has induced a bacterium to take up an entire 1.08-million-base genome in one gulp. In doing so, microbiologist John Glass and his colleagues at the J. Craig Venter Institute in Rockville, Maryland, have transformed one bacterial species into another.
Lartigue2007barticleLartigue, Carole ; Glass, John ; Alperovich, Nina ; Pieper, Rembert ; Parmar, Prashanth ; Hutchison, III Clyde ; Smith, Hamilton ; Venter, J.Genome Transplantation in Bacteria: Changing One Species to AnotherScience2007Jun281144622--1144622synthetic biology10.1126/science.1144622http://www.sciencemag.org/cgi/content/abstract/1144622As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
ref70electronicGenome transplant makes species switch - on article in Sciencesynthetic biologyhttp://www.nature.com/news/2007/070625/full/070625-9.htmlBy transplanting their genomes, US scientists have converted one species into another. John Glass and his co-workers at the J. Craig Venter Institute in Rockville, Maryland, have taken DNA from a bacterium called Mycoplasma mycoides and inserted it into cells of the closely related species Mycoplasma capricolum. They find that the recipient cells with the new genome behave like those of the donor species, making protein molecules characteristic of the donor. It's like re-booting a cell with a new operating system, says Glass.
ref71articleMeanings of 'life'Nature2007Jun2844771481031--1032synthetic biology10.1038/4471031bhttp://www.nature.com/nature/journal/v447/n7148/full/4471031b.html
Kaiser2007articleKaiser, JocelynSYNTHETIC BIOLOGY: Attempt to Patent Artificial Organism Draws a ProtestScience2007Jun1531658311557--1557synthetic biology10.1126/science.316.5831.1557http://www.sciencemag.org/cgi/content/full/316/5831/1557An activist group's concern about maverick genome sequencer J. Craig Venter's intention to patent an entirely synthetic free-living organism has thrown a spotlight on the emerging intellectual-property landscape in this hot new field. The protesters claim that Venter wants his company to become the Microsoft of synthetic biology, dominating the industry.
ref73electronicMan-made microbe 'to create endless biofuel' | Uk News | News | Telegraphsynthetic biologyhttp://www.telegraph.co.uk/news/main.jhtml?xml=/news/2007/06/08/nbiofuel108.xmlA scientist is poised to create the world's first man-made species, a synthetic microbe that could lead to an endless supply of biofuel. Craig Venter, an American who cracked the human genome in 2000, has applied for a patent at more than 100 national offices to make a bacterium from laboratory-made DNA. It is part of an effort to create designer bugs to manufacture hydrogen and biofuels, as well as absorb carbon dioxide and other harmful greenhouse gases.
Rinaudo2007barticleRinaudo, Keller ; Bleris, Leonidas ; Maddamsetti, Rohan ; Subramanian, Sairam ; Weiss, Ron ; Benenson, YaakovA universal RNAi-based logic evaluator that operates in mammalian cellsNat Biotech2007May21advanced online publicationsynthetic biology10.1038/nbt1307http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt1307.html
Dueber2007articleDueber, John ; Mirsky, Ethan ; Lim, WendellEngineering synthetic signaling proteins with ultrasensitive input/output controlNat Biotech2007Junxx256660--662synthetic biology10.1038/nbt1308http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1308.html
ref76electronicETC Group - Publications - Patenting Pandora’s Bug: Goodbye, Dolly...Hello, Synthia! J. Craig Venter Institute Seeks Monopoly Patents on the World’s First-Ever Human-Made Life Formsynthetic biologyhttp://www.etcgroup.org/en/materials/publications.html?pub_id=631The Venter Institute - named for its founder and CEO, J. Craig Venter, the scientist who led the private sector race to map the Human Genome - is applying for worldwide patents on what they refer to as ``Mycoplasma laboratorium.''
ref77electronicSecond Life, or Not? -- Kaiser 2007 (607): 3 -- ScienceNOWsynthetic biologyhttp://sciencenow.sciencemag.org/cgi/content/full/2007/607/3An activist group is raising a ruckus about what it says may be the first effort to patent an entirely synthetic free-living organism. It says the patent application, filed by maverick genome sequencer Craig Venter's institute on an idea that has likely not yet been achieved, would tie up a new technology and could aid bioterrorists. But others say there's nothing new or surprising about the patenting effort. The work involves a simple bacterium called Mycoplasma genitalium that Venter's eponymous institute in Rockville, Maryland, has been tinkering with for years. An early goal was to determine the minimum number of genes for life, and in 1999, scientists there published a rough tally. Now, they want to synthesize this ``minimal genome'' from scratch, get it working inside a cell, then add genes that would enable the bug to crank out hydrogen or ethanol to produce cheap energy.
Andrianantoandro2006barticleAndrianantoandro, Ernesto ; Basu, Subhayu ; Karig, David ; Weiss, RonSynthetic biology: new engineering rules for an emerging discipline.Molecular systems biology2006xxxx22006--0028synthetic biology10.1038/msb4100073http://www.nature.com/msb/journal/v2/n1/full/msb4100073.html
Yeh2007barticleYeh, Brian ; Rutigliano, Robert ; Deb, Anrica ; Bar-Sagi, Dafna ; Lim, WendellRewiring cellular morphology pathways with synthetic guanine nucleotide exchange factorsNature2007May314477144596--600synthetic biology10.1038/nature05851http://www.nature.com/nature/journal/vaop/ncurrent/abs/nature05851.html
ref80electronicScientists Push the Boundaries of Human Life - Newsweek: International Editions - MSNBC.comsynthetic biologyhttp://www.msnbc.msn.com/id/18882828/site/newsweek/page/0/
ref81electronicSynthetic biology--putting engineering into biology -- Heinemann and Panke 22 (22): 2790 -- Bioinformaticssynthetic biologyhttp://bioinformatics.oxfordjournals.org/cgi/content/abstract/22/22/2790
ref82electronicMain Page - IGEMsynthetic biologyhttp://parts2.mit.edu/wiki/index.php/Main_PageThe international Genetically Engineered Machine competition iGEM is an international arena where student teams compete to design and assemble engineered machines using advanced genetic components and technologies. Learn more.
ref83electronicSynBERC - Synthetic Biology Engineering Research Centersynthetic biologyhttp://www.synberc.org/index.htmlConsortium of organizations to develop synthetic biology applocations, tools and ethics. Promoted by Drew Endy and George Church. also linked to the IGEM program.
ref84articleThe Generation of New Protein Functions by the Combination of DomainsStructure2007xxxx15185--85synthetic biology10.1016/j.str.2006.11.009http://dx.doi.org/10.1016/j.str.2006.11.009
Chen2007articleChen, Chun-Hong ; Huang, Haixia ; Ward, Catherine ; Su, Jessica ; Schaeffer, Lorian ; Guo, Ming ; Hay, BruceA synthetic maternal-effect selfish genetic element drives population replacement in Drosophila.Science (New York, N.Y.)2007Apr273165824597--600synthetic biology10.1126/science. 1138595http://www.sciencemag.org/cgi/content/abstract/1138595
ref86articleA synthetic biology challenge: making cells computeMolecular BioSystems2007xxxxsynthetic biology10.1039/b618473chttp://dx.doi.org/10.1039/b618473c
ref87articleMultiple High-Throughput Analyses Monitor the Response of E. coli to PerturbationsScience2007xxxx3165824593--593Lab techniques10.1126/science.1132067http://www.sciencemag.org/cgi/content/abstract/1132067v1?etoc
ref88electronicIn situ DNA synthesis on glass substrate for microarray fabrication using self-focusing acoustic transducersynthetic biologyhttp://ieeexplore.ieee.org/search/srchabstract.jsp?arnumber=1613915&isnumber=33881&punumber=8856&k2dockey=1613915@ieeejrns&query=(+eun+sok+kim%3Cin%3Eau)&pos=3A proof of concept of using SFATs (self-focusing acoustic transducers) to print DNA oligos.
Chan2005articleChan, Leon ; Kosuri, Sriram ; Endy, DrewRefactoring bacteriophage T7.Molecular systems biology2005xxxx12005--0018refactoring10.1038/msb4100025http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16729053&query_hl=5&itool=pubmed_docsum
Zhang2007articleZhang, Feng ; Wang, Li-Ping ; Brauner, Martin ; Liewald, Jana ; Kay, Kenneth ; Watzke, Natalie ; Wood, Phillip ; Bamberg, Ernst ; Nagel, Georg ; Gottschalk, Alexander ; Deisseroth, KarlMultimodal fast optical interrogation of neural circuitryNature2007Apr054467136633--639Neurobiology10.1038/nature05744http://www.nature.com/nature/journal/v446/n7136/abs/nature05744.html
ref91electronicApplied Biodynamics Laboratory, Boston U.Labhttp://www.bu.edu/abl/Our lab is focused on developing and using nonlinear dynamics approaches to study, mimic and improve the function of biological systems. We are currently working in three areas:SB andsynrhetic biology
Andrianantoandro2006carticleAndrianantoandro, Ernesto ; Basu, Subhayu ; Karig, David ; Weiss, RonSynthetic biology: new engineering rules for an emerging discipline.Molecular systems biology [electronic resource].2006xxxx22006--0028systems biology10.1038/msb4100073http://www.nature.com/msb/journal/v2/n1/full/msb4100073.html
Heinemann2006articleHeinemann, Matthias ; Panke, SvenSynthetic biology--putting engineering into biology.Bioinformatics (Oxford, England)2006Nov1522222790--2799systems biology10.1093/bioinformatics/btl469http://dx.doi.org/10.1093/bioinformatics/btl469
Drubin2007barticleDrubin, David ; Way, Jeffrey ; Silver, PamelaDesigning biological systems.Genes \& development2007Feb01213242--254systems biology10.1101/gad.1507207http://www.genesdev.org/cgi/content/full/21/3/242
Sismour2005articleSismour, A. ; Benner, StevenSynthetic biology.Expert opinion on biological therapy2005Novxx5111409--1414systems biology10.1517/14712598.5.11.1409http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16255644&query_hl=1&itool=pubmed_DocSum
ref96electronicSpringerLink - Journal Articlesynthetic biologyhttp://www.springerlink.com/content/k7377586j4305247/
Yokobayashi2002aarticleYokobayashi, Yohei ; Weiss, Ron ; Arnold, FrancesFrom the Cover: Directed evolution of a genetic circuitProceedings of the National Academy of Sciences2002Dec24992616587--16591synthetic biology10.1073/pnas.252535999http://www.pnas.org/cgi/content/abstract/99/26/16587?etoc
ref98articleGenetic parts to program bacteriaCurrent Opinion in Biotechnology2006xxxx175548--548synthetic biology10.1016/j.copbio.2006.09.001http://dx.doi.org/10.1016/j.copbio.2006.09.001
ref99articleThe Generation of New Protein Functions by the Combination of DomainsStructure2007xxxx15185--85structures10.1016/j.str.2006.11.009http://dx.doi.org/10.1016/j.str.2006.11.009
Sprinzak2005aarticleSprinzak, David ; Elowitz, MichaelReconstruction of genetic circuitsNature2005Nov244387067443--448synthetic biology10.1038/nature04335http://www.nature.com/nature/journal/v438/n7067/full/nature04335.html
Hasty2001bookHasty, Jeff ; Isaacs, Farren ; Dolnik, Milos ; McMillen, David ; Collins, J. J.Designer Gene Networks: Towards Fundamental Cellular Control2001Mar13genetic networkhttp://arxiv.org/abs/physics/0103034
Fox2007articleFox, Richard ; Davis, S. ; Mundorff, Emily ; Newman, Lisa ; Gavrilovic, Vesna ; Ma, Steven ; Chung, Loleta ; Ching, Charlene ; Tam, Sarena ; Muley, Sheela ; Grate, John ; Gruber, John ; Whitman, John ; Sheldon, Roger ; Huisman, GjaltImproving catalytic function by ProSAR-driven enzyme evolutionNat Biotech2007Feb18advanced online publicationdirected evolution10.1038/nbt1286http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1286.html
ref103articleSynthetic biology projects in vitroGenome Research2006xxxx1711--1Cell-Free System10.1101/gr.5776007http://www.genome.org/cgi/content/abstract/gr.5776007v1?papetoc
ref104articleGenome Biology | Full text | Fast, cheap and somewhat in controlGenome Biology2006xxxx78114--114synthetic biology10.1186/gb-2006-7-8-114http://dx.doi.org/10.1186/gb-2006-7-8-114
Thattai2004articleThattai, Mukund ; Van Oudenaarden, AlexanderStochastic Gene Expression in Fluctuating EnvironmentsGenetics2004May011671523--530gene expression10.1534/genetics.167.1.523http://www.genetics.org/cgi/content/abstract/167/1/523
Li2007articleLi, Mamie ; Elledge, StephenHarnessing homologous recombination in vitro to generate recombinant DNA via SLICNat Meth2007Feb11advanced online publicationmethod10.1038/nmeth1010http://www.nature.com/nmeth/journal/vaop/ncurrent/abs/nmeth1010.html
ref107electronicETC Group - Publications - Extreme Genetic Engineering: An Introduction to Synthetic Biologytech talk 2-12-07a contrary position on some synthetic bio issues. well worth checking out to be aware of the government and world-wide issues that may shape this field's future. (smolkelab 2007-02-12 16:56:43)http://www.etcgroup.org/en/materials/publications.html?id=602* If a small circle of synthetic biologists get their way, governance of extreme genetic engineering will be left entirely in their hands. * There must be a broad societal debate on synthetic biology’s wider socio-economic and ethical implications, including potential impacts on health, environment, human rights and security. * It is not for scientists to either control public discourse or determine regulatory frameworks.
Shou2007aarticleShou, Wenying ; Ram, Sri ; Vilar, JoseFrom the Cover: Synthetic cooperation in engineered yeast populationsProceedings of the National Academy of Sciences2007Feb0610461877--1882tech talk 2-12-0710.1073/pnas.0610575104http://www.pnas.org/cgi/content/abstract/0610575104v1Cooperative interactions are key to diverse biological phenomena ranging from multicellularity to mutualism. Such diversity makes the ability to create and control cooperation desirable for potential applications in areas as varied as agriculture, pollutant treatment, and medicine. Here we show that persistent cooperation can be engineered by introducing a small set of genetic modifications into previously noninteracting cell populations. Specifically, we report the construction of a synthetic obligatory cooperative system, termed CoSMO (cooperation that is synthetic and mutually obligatory), which consists of a pair of nonmating yeast strains, each supplying an essential metabolite to the other strain. The behavior of the two strains in isolation, however, revealed unintended constraints that restrict cooperation, such as asymmetry in starvation tolerance and delays in nutrient release until near cell death. However, the joint system is shown mathematically and experimentally to be viable over a wide range of initial conditions, with oscillating population ratio settling to a value predicted by nutrient supply and consumption. Unexpectedly, even in the absence of explicitly engineered mechanisms to stabilize cooperation, the cooperative system can consistently develop increased ability to survive reductions in population density. Extending synthetic biology from the design of genetic circuits to the engineering of ecological interactions, CoSMO provides a quantitative system for linking processes at the cellular level to the collective behavior at the system level, as well as a genetically tractable system for studying the evolution of cooperation.
Drubin2007carticleDrubin, David ; Way, Jeffrey ; Silver, PamelaDesigning biological systemsGenes and Development2007Feb01213242--254review10.1101/gad.1507207http://www.genesdev.org/cgi/content/full/21/3/242
ref110articleLight-powering Escherichia coli with proteorhodopsinProceedings of the National Academy of Sciences2007xxxxenergy10.1073/pnas.0611035104http://dx.doi.org/10.1073/pnas.0611035104
Shou2007barticleShou, Wenying ; Ram, Sri ; Vilar, J. M. G.Synthetic cooperation in engineered yeast populationsProceedings of the National Academy of Sciences2007Jan310610575104--0610575104yeast10.1073/pnas.0610575104http://www.pnas.org/cgi/content/abstract/0610575104v1
Lim2007articleLim, Han ; Van Oudenaarden, AlexanderA multistep epigenetic switch enables the stable inheritance of DNA methylation statesNat Genet2007Febxx392269--275synthetic biology10.1038/ng1956http://www.nature.com/ng/journal/vaop/ncurrent/abs/ng1956.html
ref113articleSynthetic biology projects in vitroGenome Research2006xxxx1711--1synthetic biology10.1101/gr.5776007http://www.genome.org/cgi/content/abstract/gr.5776007v1?papetoc
ref114articleGenetic parts to program bacteriaCurrent Opinion in Biotechnology2006xxxx175548--548synthetic biology10.1016/j.copbio.2006.09.001http://dx.doi.org/10.1016/j.copbio.2006.09.001
Simpson2006articleSimpson, MichaelCell-free synthetic biology: a bottom-up approach to discovery by designMol Syst Biol2006Dec122Cell-Free System10.1038/msb4100104http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html
Kim2006articleKim, Jongmin ; White, Kristin ; Winfree, ErikConstruction of an in vitro bistable circuit from synthetic transcriptional switchesMol Syst Biol2006Dec122synthetic biology10.1038/msb4100099http://www.nature.com/msb/journal/v2/n1/full/msb4100099.html
ref117articleA synthetic multicellular system for programmed pattern formationNature2005xxxx43470371130--1130synthetic biology10.1038/nature03461http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15858574
ref118articleCellular Logic with Orthogonal RibosomesJournal of the American Chemical Society2005xxxx1275017584--17584synthetic biology10.1021/ja055338dhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16351070&query_hl=8&itool=pubmed_docsum
Hasty2002articleHasty, Jeff ; McMillen, David ; Collins, J.Engineered gene circuitsNature2002Nov144206912224--230genetic network10.1038/nature01257http://www.nature.com/nature/journal/v420/n6912/full/nature01257.html
ref120electronicZinc Fingers Consortiumgenetic networkhttp://www.zincfingers.org/scientific-background.htm
ref121articleStandardized reagents and protocols for engineering zinc finger nucleases by modular assemblyNature Protocols2006xxxx141637--1637genetic network10.1038/nprot.2006.259http://www.nature.com/nprot/journal/v1/n3/full/nprot.2006.259.html
ref122articleSynthetic biology--putting engineering into biologyBioinformatics2006xxxx22222790--2790synthetic biology10.1093/bioinformatics/btl469http://dx.doi.org/10.1093/bioinformatics/btl469
Seelig2006articleSeelig, Georg ; Soloveichik, David ; Zhang, David ; Winfree, ErikEnzyme-Free Nucleic Acid Logic CircuitsScience2006Dec0831458051585--1588genetic network10.1126/science.1132493http://www.sciencemag.org/cgi/content/full/314/5805/1585
Forster2006aarticleForster, Anthony ; Church, GeorgeSynthetic biology projects in vitroGenome Research2006Dec065776007--5776007synthetic biology10.1101/gr.5776007http://www.genome.org/cgi/content/abstract/gr.5776007v1?papetoc
Atsumi2006articleAtsumi, Shota ; Little, JohnA synthetic phage \{lambda\} regulatory circuitProceedings of the National Academy of Sciences2006Nov290603052103--0603052103synthetic biology10.1073/pnas.0603052103http://www.pnas.org/cgi/content/abstract/0603052103v1?etoc
ref126electronicThe New Atlantis - The Promise and Perils of Synthetic Biology - Jonathan B. Tucker and Raymond A. Zilinskassynthetic biologyhttp://www.thenewatlantis.com/archive/12/tuckerzilinskas.htm
ref127electronicOptimizing Biological Circuits: Integrating Rational Design with Directed Evolutionsynthetic biologyhttp://aiche.confex.com/aiche/2006/preliminaryprogram/abstract_52714.htm
Collins2005articleCollins, Cynthia ; Arnold, Frances ; Leadbetter, JaredDirected evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactonesMolecular Microbiology2005xxxx553712--723synthetic biology10.1111/j.1365-2958.2004.04437.xhttp://www.blackwell-synergy.com/links/doi/10.1111/j.1365-2958.2004.04437.x/enhancedabs/
Vilar2006articleVilar, JoseModularizing gene regulationMol Syst Biol2006Mar212gene expression10.1038/msb4100058http://www.nature.com/msb/journal/v2/n1/full/msb4100058.html
Wolf2003articleWolf, Denise ; Arkin, AdamMotifs, modules and games in bacteria.Current opinion in microbiology.2003Aprxx62125--134synthetic biologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12732301&dopt=Abstract
Michnick2006articleMichnick, StephenA luxRury of synthetic signalsNat Biotech2006Junxx246658--660pLuxR10.1038/nbt0606-658http://www.nature.com/nbt/journal/v24/n6/full/nbt0606-658.html
Cookson2005articleCookson, Scott ; Ostroff, Natalie ; Pang, Wyming ; Volfson, Dmitri ; Hasty, AndMonitoring dynamics of single-cell gene expression over multiple cell cyclesMol Syst Biol2005Nov22114100032--4100031thesis\_chapter\_210.1038/msb4100032http://www.nature.com/msb/journal/v1/n1/full/msb4100032.html
Anderson2006articleAnderson, J. ; Clarke, Elizabeth ; Arkin, Adam ; Voigt, ChristopherEnvironmentally controlled invasion of cancer cells by engineered bacteria.Journal of molecular biology.2006Jan273554619--627synthetic biology10.1016/j.jmb.2005.10.076http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16330045&dopt=Citation
ref134electronicTuning genetic control through promoter engineering -- Alper et al. 102 (36): 12678 -- Proceedings of the National Academy of Sciencessynthetic biologyhttp://www.pnas.org/cgi/content/abstract/102/36/12678
Schibler2005articleSchibler, Ueli ; Naef, FelixCellular oscillators: rhythmic gene expression and metabolism.Current opinion in cell biology.2005Aprxx172223--229synthetic biology10.1016/j.ceb.2005.01.007http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15780601&dopt=Citation
Atsumi2004articleAtsumi, Shota ; Little, JohnRegulatory circuit design and evolution using phage \{lambda\}Genes and Development2004Sep0118172086--2094synthetic biology10.1101/gad.1226004http://www.genesdev.org/cgi/content/abstract/18/17/2086
Balagadde2005articleBalagadde, Frederick ; You, Lingchong ; Hansen, Carl ; Arnold, Frances ; Quake, StephenLong-Term Monitoring of Bacteria Undergoing Programmed Population Control in a MicrochemostatScience2005Jul013095731137--140synthetic biology10.1126/science.1109173http://www.sciencemag.org/cgi/content/abstract/309/5731/137
ref138electronicThe Device Physics of Cellular Logic Gates - Weiss, Basu (ResearchIndex)synthetic biologyhttp://citeseer.ist.psu.edu/weiss02device.html
Ehrenberg2003articleEhrenberg, Mans ; Elf, Johan ; Aurell, Erik ; Sandberg, Rickard ; Tegner, AndSystems Biology Is Taking OffGenome Research2003Nov0113112377--2380thesis\_chapter\_1http://www.genome.org/cgi/content/full/13/11/2377
Hooshangi2005barticleHooshangi, Sara ; Thiberge, Stephan ; Weiss, AndUltrasensitivity and noise propagation in a synthetic transcriptional cascadeProceedings of the National Academy of Sciences2005Mar08102103581--3586thesis\_chapter\_210.1073/pnas.0408507102http://dx.doi.org/10.1073/pnas.0408507102
Bulter2004articleBulter, Thomas ; Lee, Sun-Gu ; Wong, Wilson ; Fung, Eileen ; Connor, Michael ; Liao, JamesFrom the Cover: Design of artificial cell-cell communication using gene and metabolic networksProceedings of the National Academy of Sciences2004Feb2410182299--2304synthetic biology10.1073/pnas.0306484101http://www.pnas.org/cgi/content/abstract/pnas;101/8/2299
You2004articleYou, Lingchong ; Sidney-Cox, Robert ; Weiss, Ron ; Arnold, AndProgrammed population control by cell-cell communication and regulated killingNature2004Apr224286985868--871thesis\_chapter\_110.1038/nature02491http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15064770
Weiss2003articleWeiss, Ron ; Basu, Subhayu ; Hooshangi, Sara ; Kalmbach, Abigail ; Karig, David ; Mehreja, Rishabh ; Netravali, IlkaGenetic circuit building blocks for cellular computation, communications, and signal processingNatural Computing2003xxxx2147--47thesis\_chapter\_110.1023/a:1023307812034http://dx.doi.org/10.1023/a:1023307812034
McAdams2000articleMcAdams, H. H. ; Arkin, A.Towards a circuit engineering discipline.Current biology : CB.2000Apr20108318--320synthetic biologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10801411&dopt=Abstract
McMillen2002articleMcMillen, David ; Kopell, Nancy ; Hasty, Jeff ; Collins, J. J.Synchronizing genetic relaxation oscillators by intercell signalingProceedings of the National Academy of Sciences2002Jan22992679--684application10.1073/pnas.022642299http://www.pnas.org/cgi/content/abstract/99/2/679
Kobayashi2004articleKobayashi, Hideki ; Kaern, Mads ; Araki, Michihiro ; Chung, Kristy ; Gardner, Timothy ; Cantor, Charles ; Collins, JamesProgrammable cells: Interfacing natural and engineered gene networksProceedings of the National Academy of Sciences2004Jun01101228414--8419cell-cell signaling10.1073/pnas.0402940101http://www.pnas.org/cgi/content/abstract/101/22/8414?etoc
Tegner2003articleTegner, Jesper ; Yeung, M. K. ; Hasty, Jeff ; Collins, JamesReverse engineering gene networks: Integrating genetic perturbations with dynamical modelingProceedings of the National Academy of Sciences2003May13100105944--5949synthetic biology10.1073/pnas.0933416100http://www.pnas.org/cgi/content/full/100/10/5944?ijkey=8ceb79f77cfcaade8d7b71bbdafdd0e60853460e&keytype2=tf_ipsecsha
Alm2003articleAlm, Eric ; Arkin, AdamBiological networks.Current opinion in structural biology.2003Aprxx132193--202synthetic biologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=12727512&dopt=Citation
Isaacs2003articleIsaacs, Farren ; Hasty, Jeff ; Cantor, Charles ; Collins, J. J.Prediction and measurement of an autoregulatory genetic moduleProceedings of the National Academy of Sciences2003Jun24100137714--7719thesis\_chapter\_210.1073/pnas.1332628100http://www.pnas.org/cgi/content/abstract/100/13/7714
Yokobayashi2002barticleYokobayashi, Yohei ; Weiss, Ron ; Arnold, FrancesDirected evolution of a genetic circuitProceedings of the National Academy of Sciences2002Dec24992616587--16591synthetic biology10.1073/pnas.252535999http://www.pnas.org/cgi/content/abstract/99/26/16587?etoc
ref151bookDunlop, Mary ; Murray, RichardTowards Biological System Identification: Fast and Accurate Estimatessynthetic biologyhttp://www.its.caltech.edu/~mjdunlop/papers/CDC06-draft.pdf
Pottage2006articlePottage, AlainToo Much Ownership: Bio-prospecting in the Age of Synthetic BiologyBioSocieties. Cambridge University Press2006Junxx137--158synthetic biology10.1017/S1745855206050241http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=446754
Swain2006articleSwain, Peter ; Longtin, AndreNoise in genetic and neural networksChaos: An Interdisciplinary Journal of Nonlinear Science2006xxxx162026101--026101synthetic biologyhttp://link.aip.org/link/?CHA/16/026101/1
McDaniel2005articleMcDaniel, Ryan ; Weiss, RonAdvances in synthetic biology: on the path from prototypes to applications.Current opinion in biotechnology.2005Augxx164476--483thesis\_chapter\_110.1016/j.copbio.2005.07.002http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16019200
ref155articleKeasling, JayEngineering Bacteria for Drug ProductionEngineering Bacteria for Drug Productionsynthetic biologyhttp://www.nae.edu/nae/naefoe.nsf/0754c87f163f599e85256cca00588f49/85256dad0044ef2a852570bd00521b49/$FILE/Keasling.pdf
Blake2004articleBlake, William ; Isaacs, FarrenSynthetic biology evolves.Trends in biotechnology.2004Julxx227321--324synthetic biology10.1016/j.tibtech.2004.04.008http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15245901&dopt=Citation
Ray2003articleRay, ThomasAn evolutionary approach to synthetic biology: zen in the art of creating lifeAdvances in evolutionary computing: theory and applications2003xxxx479--517synthetic biologyhttp://portal.acm.org/citation.cfm?id=903758.903778
Pfleger2006aarticlePfleger, Brian ; Pitera, Douglas ; Smolke, Christina ; Keasling, AndCombinatorial engineering of intergenic regions in operons tunes expression of multiple genesNat Biotech2006Augxx2481027--1032synthetic biology10.1038/nbt1226http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1226.html
Check2005barticleCheck, ErikaSynthetic biology: Designs on lifeNature2005Nov244387067417--418synthetic biology10.1038/438417ahttp://www.nature.com/nature/journal/v438/n7067/full/438417a.html
Andrianantoandro2006darticleAndrianantoandro, Ernesto ; Basu, Subhayu ; Karig, David ; Weiss, AndSynthetic biology: new engineering rules for an emerging disciplineMol Syst Biol2006xxxx24100073--4100071synthetic biology10.1038/msb4100073http://www.nature.com/msb/journal/v2/n1/full/msb4100073.html
Endy2005aarticleEndy, DrewFoundations for engineering biologyNature2005Nov244387067449--453synthetic biology10.1038/nature04342http://www.nature.com/nature/journal/v438/n7067/full/nature04342.html
Ball2004articleBall, PhilipSynthetic biology: Starting from scratchNature2004Oct074317009624--626synthetic biology10.1038/431624ahttp://www.nature.com/nature/journal/v431/n7009/full/431624a.html
Elowitz2000aarticleElowitz, Michael ; Leibler, StanislasA synthetic oscillatory network of transcriptional regulatorsNature2000Jan204036767335--338synthetic biology10.1038/35002125http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10659856&query_hl=7
Brent2004articleBrent, RogerA partnership between biology and engineeringNat Biotech2004Octxx22101211--1214thesis\_chapter\_110.1038/nbt1004-1211http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15470452
Isaacs2006articleIsaacs, Farren ; Dwyer, Daniel ; Collins, AndRNA synthetic biologyNat Biotech2006Mayxx245545--554riboswitch10.1038/nbt1208http://dx.doi.org/10.1038/nbt1208
Fu2006aarticleFu, PengchengA perspective of synthetic biology: assembling building blocks for novel functions.Biotechnology journal.2006Junxx16690--699review10.1002/biot.200600019http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16892318&itool=pubmed_DocSum
Benner2005barticleBenner, Steven ; Sismour, A.Synthetic biology.Nature reviews. Genetics.2005Julxx67533--543review10.1038/nrg1637http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15995697
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Chin2006aarticleChin, Jason W.Programming and engineering biological networks.Current opinion in structural biology.2006Augxx164551--556genetic10.1016/j.sbi.2006.06.0110959-440Xhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16842994&itool=pubmed_DocSum
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ref174electronicLibrary of Synthetic 5' Secondary Structures To Manipulate mRNA Stability in Escherichia colisynthetic biologyhttp://pubs.acs.org/cgi-bin/abstract.cgi/bipret/1999/15/i01/abs/bp9801143.html
Arraiano2003articleArraiano, Cecilia Maria ; Maquat, Lynne ElizabethPost-transcriptional control of gene expression: effectors of mRNA decayMolecular Microbiology2003xxxx491267--276synthetic biology10.1046/j.1365-2958.2003.03545.xhttp://www.blackwell-synergy.com/links/doi/10.1046/j.1365-2958.2003.03545.xDescription of various mechanisms of mRNA degredation
ref176electronicA comprehensive library of fluorescent transcriptional reporters for Escherichia colisynthetic biologyhttp://www.weizmann.ac.il/mcb/UriAlon/Papers/Zaslaver_Ecoli_library.pdf
ref177electronicSynthetic biology | Life 2.0 | Economist.comsynthetic biologyhttp://www.economist.com/business/displaystory.cfm?story_id=7854314
ref178electronicSynthetic Biologysynthetic biologyhttp://syntheticbiology.org/
Maeda2006aarticleMaeda, Yusuke T. ; Sano, MasakiRegulatory dynamics of synthetic gene networks with positive feedback.Journal of molecular biology.2006Jun1635941107--1124synthetic biology10.1016/j.jmb.2006.03.0640022-2836http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16701695&query_hl=12&itool=pubmed_docsum
ref180electronicCell -- Banaszynski et al.synthetic biologyhttp://www.cell.com/content/article/fulltext?uid=PIIS0092867406010130
ref181electronicSynthetic biology | Playing demigods | Economist.comsynthetic biologyhttp://www.economist.com/opinion/displaystory.cfm?story_id=E1_SRVQSST
ref182electronicSynthetic biology | Life 2.0 | Economist.comsynthetic biologyhttp://www.economist.com/business/displaystory.cfm?story_id=7854314
Church2005barticleChurch, GeorgeFrom systems biology to synthetic biologyMol Syst Biol2005Mar2911--1synthetic biology10.1038/msb4100007http://www.nature.com/msb/journal/v1/n1/full/msb4100007.html#b5
Forster2006barticleForster, Anthony ; Church, GeorgeTowards synthesis of a minimal cellMol Syst Biol2006Aug222synthetic biology10.1038/msb4100090http://www.nature.com/msb/journal/v2/n1/full/msb4100090.html
Forster2006carticleForster, Anthony ; Church, GeorgeTowards synthesis of a minimal cellMol Syst Biol2006Aug222synthetic biology10.1038/msb4100090http://www.nature.com/msb/journal/v2/n1/full/msb4100090.html
Lim2006articleLim, Kwang-il ; Lang, Tobias ; Lam, Vy ; Yin, JohnModel-Based Design of Growth-Attenuated VirusesPLoS Computational Biology2006Sep0129e116systems biology10.1371/journal.pcbi.0020116http://compbiol.plosjournals.org/perlserv/?request=get-document&doi=10.1371%2Fjournal.pcbi.0020116
ref187electronicSynthetic biology | Bedroom biotech | Economist.comsynthetic biologyhttp://www.economist.com/displayStory.cfm?Story_id=7854327Like information technology before it, biotech is starting to spawn hackers
ref188electronicSynthetic biology | Life 2.0 | Economist.comsynthetic biologyhttp://www.economist.com/displayStory.cfm?Story_id=7854314The new science of synthetic biology is poised between hype and hope. But its time will soon come. Describes this vein of science being on an innovation curve quite similar to Moore's law. Discusses BioBricks and work by Rob Carlson.
ref189electronicSynthetic biology | Playing demigods | Economist.comethicshttp://www.economist.com/research/articlesBySubject/displayStory.cfm?story_id=7854771&subjectid=526354Discusses innovation, possibilities and fears of synthetic biology. Recommends caution and monitoring, but not shut down.
Fu2006barticleFu, PengchengA perspective of synthetic biology: Assembling building blocks for novel functions.Biotechnology journal.2006Junxx16690--699synthetic biology10.1002/biot.2006000191860-6768http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16892318&itool=pubmed_DocSum
Maeda2006barticleMaeda, Yusuke T. ; Sano, MasakiRegulatory dynamics of synthetic gene networks with positive feedback.Journal of molecular biology.2006Jun1635941107--1124synthetic biology10.1016/j.jmb.2006.03.0640022-2836http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16701695&query_hl=12&itool=pubmed_docsum
Chin2006barticleChin, Jason W.Programming and engineering biological networks.Current opinion in structural biology.2006Augxx164551--556gene10.1016/j.sbi.2006.06.0110959-440Xhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16842994&itool=pubmed_DocSum
Pfleger2006barticlePfleger, Brian ; Pitera, Douglas ; Smolke, Christina ; Keasling, JayCombinatorial engineering of intergenic regions in operons tunes expression of multiple genesNature Biotechnology2006Jul16advanced online publication81027--1032synthetic biology10.1038/nbt122610870156http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1226.html
Pfleger2006carticlePfleger, Brian ; Pitera, Douglas ; Smolke, Christina ; Keasling, JayCombinatorial engineering of intergenic regions in operons tunes expression of multiple genesNature Biotechnology2006Jul16advanced online publication81027--1032synthetic biology10.1038/nbt122610870156http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1226.html
Posfai2006articlePosfai, Gyorgy ; Plunkett, Guy III ; Feher, Tamas ; Frisch, David ; Keil, Gunther M. ; Umenhoffer, Kinga ; Kolisnychenko, Vitaliy ; Stahl, Buffy ; Sharma, Shamik S. ; de Arruda, Monika ; Burland, Valerie ; Harcum, Sarah W. ; Blattner, Frederick R.Emergent Properties of Reduced-Genome Escherichia coliScience2006May1931257761044--1046emergencehttp://www.sciencemag.org/cgi/content/abstract/312/5776/1044?etoc
Villalobos2006articleVillalobos, Alan ; Ness, Jon ; Gustafsson, Claes ; Minshull, Jeremy ; Govindarajan, SridharGene Designer: A Synthetic Biology Tool for Constructing Artificial DNA Segments.BMC Bioinformatics2006Jun0671285synthetic biology10.1186/1471-2105-7-2851471-2105http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16756672&itool=pubmed_DocSum
Andrianantoandro2006earticleAndrianantoandro, Ernesto ; Basu, Subhayu ; Karig, David ; Weiss, RonSynthetic biology: new engineering rules for an emerging discipline.Molecular systems biology [electronic resource].2006xxxx2msb4100073--0028synthetic biology10.1038/msb41000731744-4292http://www.nature.com/msb/journal/v2/n1/full/msb4100073.html
Xiang2006articleXiang, Shuanglin ; Fruehauf, Johannes ; Li, ChiangShort hairpin RNA-expressing bacteria elicit RNA interference in mammalsNature Biotechnology2006May14advanced online publication6697--702synthetic biology10.1038/nbt121110870156http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1211.htmlNonpathogenic Escherichia coli were engineered to transcribe shRNAs from a plasmid containing the invasin gene Inv and the listeriolysin O gene HlyA, which encode two bacterial factors needed for successful transfer of the shRNAs into mammalian cells.
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Church2005carticleChurch, GeorgeFrom systems biology to synthetic biologyMolecular systems biology [electronic resource].2005Mar2911msb4100007--0032synthetic biology10.1038/msb41000071744-4292http://www.nature.com/msb/journal/v1/n1/full/msb4100007.html#b5editorial
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Endy2005barticleEndy, DrewFoundations for engineering biologyNature2005Nov244387067449--453synthetic biology10.1038/nature043420028-0836http://www.nature.com/nature/journal/v438/n7067/full/nature04342.html
ref203electronicOpenWetWaresynthetic biologyhttp://openwetware.org/wiki/Main_Pagesee the comic Adventures in synthetic biology
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Benner2003articleBenner, StevenSynthetic biology: Act naturalNature2003Jan094216919118nature concepts10.1038/421118a0028-0836http://www.nature.com/nature/journal/v421/n6919/full/421118a.html
ref206electronicEngineering Life: Building a Fab for Biology -- [ BIOTECHNOLOGY ] -- Principles and practices learned from engineering successes can help transform biotechnology from a specialized craft into a mature industrysynthetic biologyBy the Bio FAB group (ht265 2006-06-06 13:24:51)http://www.sciam.com/article.cfm?chanID=sa006&colID=1&articleID=000E491E-B2F9-146C-B2F983414B7F0000Synthetic Biology by Baker
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Morton2006aarticleMorton, OliverSynthetic Biology 2.0: More bloggersMainlyMartian2006May23synthetic biologyhttp://mainlymartian.blogs.com/semijournal/2006/05/synthetic_biolo_6.html
ref209articleDomains, Motifs, and Scaffolds: The Role of Modular Interactions in the Evolution and Wiring of Cell Signaling CircuitsAnnual review of biochemistry.2005xxxx75060407064831004--680synthetic biology10.1146/annurev.biochem.75.103004.1427100066-4154http://dx.doi.org/10.1146/annurev.biochem.75.103004.142710
Chin2006carticleChin, JasonModular approaches to expanding the functions of living matterNature chemical biology.2006Junxx26304--311synthetic biology10.1038/nchembio7891552-4450http://www.nature.com/nchembio/journal/v2/n6/abs/nchembio789.html
Morton2006barticleMorton, OliverSynthetic Biology 2.0: Medicine (and its malcontents?)MainlyMartian2006May22synthetic biologyhttp://mainlymartian.blogs.com/semijournal/2006/05/synthetic_biolo_5.html
Morton2006carticleMorton, OliverSynthetic Biology 2.0: Also blogging the meeting...MainlyMartian2006May21synthetic biologyhttp://mainlymartian.blogs.com/semijournal/2006/05/syhtetic_biolog.html
Morton2006darticleMorton, OliverSynthetic Biology 2.0: Opposing viewsMainlyMartian2006May20synthetic biologyhttp://mainlymartian.blogs.com/semijournal/2006/05/synthetic_biolo_3.html
Morton2006earticleMorton, OliverSynthetic Biology 2.0: Fun to comeMainlyMartian2006May20synthetic biologyhttp://mainlymartian.blogs.com/semijournal/2006/05/synthetic_biolo_2.html
Morton2006farticleMorton, OliverSynthetic Biology 2.0: BTUs per bushelMainlyMartian2006May20synthetic biologyhttp://mainlymartian.blogs.com/semijournal/2006/05/synthetic_biolo_1.html
Morton2006garticleMorton, OliverSynthetic Biology 2.0: What's afoot?MainlyMartian2006May20synthetic biologyhttp://mainlymartian.blogs.com/semijournal/2006/05/synthetic_biolo.html
Andrianantoandro2006farticleAndrianantoandro, Ernesto ; Basu, Subhayu ; Karig, David ; Weiss, RonSynthetic biology: new engineering rules for an emerging disciplineMolecular systems biology [electronic resource].2006xxxx2msb4100073--0028synthetic biology10.1038/msb41000731744-4292http://www.nature.com/msb/journal/v2/n1/full/msb4100073.html
Endy2005carticleEndy, DrewFoundations for engineering biologyNature2005Nov244387067449--453fun10.1038/nature043420028-0836http://www.nature.com/nature/journal/v438/n7067/full/nature04342.html
Elowitz2000barticleElowitz, M. B. ; Leibler, S.A synthetic oscillatory network of transcriptional regulators.Nature.2000Jan204036767335--338synthetic biology10.1038/350021250028-0836http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10659856&query_hl=7
Guet2002aarticleGuet, Călin C. ; Elowitz, Michael B. ; Hsing, Weihong ; Leibler, StanislasCombinatorial synthesis of genetic networks.Science.2002May2429655721466--1470synthetic biology10.1126/science.10674071095-9203http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12029133&query_hl=7
Levskaya2005aarticleLevskaya, Anselm ; Chevalier, Aaron ; Tabor, Jeffrey ; Simpson, Zachary Booth ; Lavery, Laura ; Levy, Matthew ; Davidson, Eric ; Scouras, Alexander ; Ellington, Andrew ; Marcotte, Edward ; Voigt, ChristopherSynthetic biology: Engineering Escherichia coli to see lightNature.2005Nov244387067441--442synthetic biology10.1038/nature044051476-4687http://www.nature.com/nature/journal/v438/n7067/abs/nature04405.html
Bray1995articleBray, DennisProtein molecules as computational elements in living cellsNature.1995Jul273766538307--312synthetic biology10.1038/376307a00028-0836http://www.nature.com/nature/journal/v376/n6538/abs/376307a0.html
Pawson1995articlePawson, TonyProtein modules and signalling networksNature1995Feb163736515573--580synthetic biology10.1038/373573a0http://www.nature.com/nature/journal/v373/n6515/abs/373573a0.html
ref224articleBioMed Central | Abstract | 1471-2105-7-55 | PathSys: integrating molecular interaction graphs for systems biologyBMC Bioinformatics2006Feb077155synthetic biology10.1186/1471-2105-7-5514712105http://dx.doi.org/10.1186/1471-2105-7-55
ref225electronicSynthetic Biology in Practicesynthetic biologyhttp://blog.lib.umn.edu/sali0090/synbio/
ref226electronicGeneral Review Papers - IGEMsynthetic biologyhttp://parts.mit.edu/wiki/index.php/General_Review_Papers
ref227electronicSynthetic systems biologysynthetic biologyhttp://www.ncbs.res.in/~faculty/ssb/apply1.html
Boonyarattanakalin2006articleBoonyarattanakalin, Siwarutt ; Athavankar, Sonalee ; Sun, Qi ; Peterson, Blake R.Synthesis of an Artificial Cell Surface Receptor that Enables Oligohistidine Affinity Tags to Function as Metal-Dependent Cell-Penetrating Peptides.Journal of the American Chemical Society.2006Jan181282386--387synthetic biology10.1021/ja056126j0002-7863http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16402806&query_hl=1&itool=pubmed_docsum
Rackham2005articleRackham, Oliver ; Chin, Jason W.A network of orthogonal ribosome x mRNA pairs.Nature chemical biology.2005Augxx13159--166synthetic biology10.1038/nchembio7191552-4450http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16408021&query_hl=1&itool=pubmed_docsum
Volgraf2006articleVolgraf, Matthew ; Gorostiza, Pau ; Numano, Rika ; Kramer, Richard H. ; Isacoff, Ehud Y. ; Trauner, DirkAllosteric control of an ionotropic glutamate receptor with an optical switch.Nature chemical biology.2006Janxx2147--52synthetic biology10.1038/nchembio7561552-4450http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16408092&query_hl=1&itool=pubmed_docsum
Simpson2004aarticleSimpson, Michael L.Rewiring the cell: synthetic biology moves towards higher functional complexity.Trends Biotechnol2004Novxx2211555--557synthetic biology10.1016/j.tibtech.2004.09.0030167-7799http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15491797&query_hl=4
ref232electronicResearch Papers in Synthetic Biology - IGEMsynthetic biologyhttp://parts.mit.edu/wiki/index.php/Research_Papers_in_Synthetic_Biology
ref233electronicRegistry of Standard Biological Partssynthetic biologyhttp://parts.mit.edu/
ref234electronicMain Page - IGEMsynthetic biologyhttp://parts.mit.edu/wiki/index.php/Main_Page
ref235electronicSignOnSanDiego.com > News > Science -- Command performancessynthetic biologyhttp://www.signonsandiego.com/news/science/20051214-9999-lz1c14circuit.html#``Controlling organisms with biological circuits, opens up a world of possibilities and dangers''
Chen2005aarticleChen, Ming-Tang ; Weiss, RonArtificial cell-cell communication in yeast Saccharomyces cerevisiae using signaling elements from Arabidopsis thalianaNature biotechnology.2005Nov20advanced online publication121551--1555synthetic biology10.1038/nbt11621087-0156http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt1162.html
Fung2005articleFung, Eileen ; Wong, Wilson ; Suen, Jason ; Bulter, Thomas ; Lee, Sun-gu ; Liao, JamesA synthetic gene-metabolic oscillatorNature2005May054357038118--122synthetic biology10.1038/nature035080028-0836http://www.nature.com/nature/journal/v435/n7038/abs/nature03508.html
Endy2005darticleEndy, DrewFoundations for engineering biologyNature2005Nov244387067449--453synthetic biology10.1038/nature043420028-0836http://www.nature.com/nature/journal/v438/n7067/full/nature04342.htmlEngineered biological systems have been used to manipulate information, construct materials, process chemicals, produce energy, provide food, and help maintain or enhance human health and our environment. Unfortunately, our ability to quickly and reliably engineer biological systems that behave as expected remains quite limited. Foundational technologies that make routine the engineering of biology are needed. Vibrant, open research communities and strategic leadership are necessary to ensure that the development and application of biological technologies remains overwhelmingly constructive.
Levskaya2005barticleLevskaya, Anselm ; Chevalier, Aaron ; Tabor, Jeffrey ; Simpson, Zachary Booth ; Lavery, Laura ; Levy, Matthew ; Davidson, Eric ; Scouras, Alexander ; Ellington, Andrew ; Marcotte, Edward ; Voigt, ChristopherSynthetic biology: Engineering Escherichia coli to see lightNature.2005Nov244387067441--442synthetic biology10.1038/nature044051476-4687http://www.nature.com/nature/journal/v438/n7067/abs/nature04405.html
Endy2005earticleEndy, DrewFoundations for engineering biologyNature2005Nov244387067449--453synthetic biology10.1038/nature043420028-0836http://www.nature.com/nature/journal/v438/n7067/full/nature04342.html
Sprinzak2005barticleSprinzak, David ; Elowitz, MichaelReconstruction of genetic circuitsNature2005Nov244387067443--448synthetic biology10.1038/nature043350028-0836http://www.nature.com/nature/journal/v438/n7067/full/nature04335.html
Levskaya2005carticleLevskaya, Anselm ; Chevalier, Aaron ; Tabor, Jeffrey ; Simpson, Zachary Booth ; Lavery, Laura ; Levy, Matthew ; Davidson, Eric ; Scouras, Alexander ; Ellington, Andrew ; Marcotte, Edward ; Voigt, ChristopherSynthetic biology: Engineering Escherichia coli to see light - article in NatureNature.2005Nov244387067441--442Photography10.1038/nature044051476-4687http://www.nature.com/nature/journal/v438/n7067/abs/nature04405.htmlWe have designed a bacterial system that is switched between different states by red light. The system consists of a synthetic sensor kinase that allows a lawn of bacteria to function as a biological film, such that the projection of a pattern of light on to the bacteria produces a high-definition (about 100 megapixels per square inch), two-dimensional chemical image. This spatial control of bacterial gene expression could be used to 'print' complex biological materials, for example, and to investigate signalling pathways through precise spatial and temporal control of their phosphorylation steps.
ref243electronicBacterial film : NaturePhotographyhttp://www.nature.com/nature/journal/v438/n7067/edsumm/e051124-07.htmlPhytochromes are membrane-bound photoreceptors found in plants and some bacteria. There are none in Escherichia coli, but with the introduction of a genetic circuit that fuses a cyanobacterial photoreceptor to an intracellular kinase, E. coli sees the light. The bacteria then act as a photographic film, producing a chemical image when light is projected onto it.
ref244electronicGuardian Unlimited || Super-sensitive film made from living bacteria - on article in NaturePhotographyhttp://www.guardian.co.uk/science/story/0,3605,1649439,00.htmlScientists have created a new kind of photographic film, made from living bacteria, which is able to detect nano-scale features and print ultra-detailed images. By genetically engineering a common form of bacterium they were able to make it switch its pigment production on and off in response to a light.
Endy2005farticleEndy, DrewFoundations for engineering biologyNature2005Nov244387067449--453synthetic biology10.1038/nature043420028-0836http://www.nature.com/nature/journal/v438/n7067/pdf/nature04342.pdf
ref246electronicBacteria can take pictures of themselves - Boston.com - on article in Naturesynthetic biologyhttp://www.boston.com/news/science/articles/2005/11/23/bacteria_can_take_pictures_of_themselves/The notorious E. coli bug made its film debut Wednesday. That's when researchers at the University of California, San Francisco and the University of Texas announced in the journal Nature that they had created photographs of themselves by programming the bacteria -- best known for outbreaks of food poisoning -- to make pictures in much the same way Kodak film produces images.
Chen2005barticleChen, Ming-Tang ; Weiss, RonArtificial cell-cell communication in yeast Saccharomyces cerevisiae using signaling elements from Arabidopsis thalianaNature biotechnology.2005Nov20advanced online publication121551--1555synthetic biology10.1038/nbt11621087-0156http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt1162.html
Pennisi2005articlePennisi, ElizabethSYNTHETIC BIOLOGY: Synthetic Biology Remakes Small GenomesScience2005Nov043105749769--770synthetic biologyhttp://www.sciencemag.org/cgi/content/full/310/5749/769
ref249electronicQualitative Reasoning in biology - symposium collectionsynthetic biologyhttp://monet.aber.ac.uk:8080/monet/docs/pdf_files/aime_03/mbqr-aime03.pdf
ref250electronicWelcome to Synthetic and Systems Biologysynthetic biologyhttp://www.ssbjournal.com/Journal Homepage
ref251articleCombining two genomes in one cell: Stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genomeProceedings of the National Academy of Sciences of the United States of America2005Nov011024415971--15976synthetic biology10.1073/pnas.05038681020027-8424http://dx.doi.org/10.1073/pnas.0503868102
ref252electronicVIROLOGY: Resurrected Influenza Virus Yields Secrets of Deadly 1918 Pandemic -- Kaiser 310 (5745): 28 -- ScienceAvianFluhttp://www.sciencemag.org/cgi/content/summary/310/5745/28
Simpson2004barticleSimpson, Michael L.Rewiring the cell: synthetic biology moves towards higher functional complexity.Trends Biotechnol2004Novxx2211555--557synthetic biology10.1016/j.tibtech.2004.09.0030167-7799http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15491797&query_hl=4Nice overview of the present state of the field and some of its future research goals
Pedraza2005barticlePedraza, Juan M. ; van Oudenaarden, AlexanderNoise propagation in gene networks.Science (New York, N.Y.)2005Mar2530757171965--1969Synthetic10.1126/science.11090901095-9203http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15790857&query_hl=12
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