Table of c4.0 Working Stocks

Working Stocks with Aptamers

Stock ID

Experiment

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

c4.0A

no latches, no aptamers

x

x

x

x

x

x

x

x

c4.0B

latch design 1, no aptamers

x

x

x

x

x

x

x

x

c4.0C

latch design 2, no aptamers

x

x

x

x

x

x

x

x

x

x

x

c4.0D

no latches, inside aptamers

x

x

x

x

x

x

x

x

c4.0E

no latches, outside aptamers

x

x

x

x

x

x

x

x

c4.0F

latch design 1, inside aptamers

x

x

x

x

x

x

x

x

c4.0G

latch design 1, outside aptamers

x

x

x

x

x

x

x

x

c4.0H

latch design 2, inside aptamers

x

x

x

x

x

x

x

x

x

x

x

c4.0I

latch design 2, outside aptamers

x

x

x

x

x

x

x

x

x

x

x

c4.0J

displace latch design 1

x

x

x

c4.0K

displace latch design 2

x

x

x

Working Stocks for Biotinylated Oligos

Stock ID

Experiment

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

4b

6b

c4.0Fb

latch design 1, inside biotin

x

x

x

x

x

x

x

x

c4.0Gb

latch design 1, outside biotin

x

x

x

x

x

x

x

x

c4.0Hb

latch design 2, inside biotin

x

x

x

x

x

x

x

x

x

x

x

c4.0Ib

latch design 2, outside biotin

x

x

x

x

x

x

x

x

x

x

x

Preworking Stocks

Description	Plate Locations	Total
c4.0.1: core barrel oligos	box 4_A: row A (1-12), row B (1-12), row C (1-12), row D (1-12), row E (1-12), row F (1-6)	66
c4.0.2: core top lid oligos	box 4_A: row F (7-12), row G (1-12), row H (1-12); box 4_B: row A (1-3)	33
c4.0.3: core bottom lid oligos	box 4_B: row A (4-12), row B (1-12), row C (1-12)	33
c4.0.4: barrel oligos @ outside aptamers -aptamers	box 4_B: row D (1-4)	4
c4.0.5: barrel oligos @ outside aptamers +aptamers	box 4_B: row D (5-8)	4
c4.0.6: barrel oligos @ inside aptamers -aptamers	box 4_B: row D (9-12)	4
c4.0.7: barrel oligos @ inside aptamers +aptamers	box 4_B: row E (1-4)	4
c4.0.8: two-oligo latch sequences for barrel	box 4_B: row E (5-8)	4
c4.0.9: four-oligo latch sequences for barrel	box 4_B: row E (9-12)	4
c4.0.10: barrel oligos @ latches -latches	box 4_B: row F (1-4)	4
c4.0.11: two-oligo latch sequences for top lid	box 4_B: row F (5-6)	2
c4.0.12: two-oligo latch sequences for bottom lid	box 4_B: row F (7-8)	2
c4.0.13: four-oligo latch sequences for top lid	box 4_C: row A (1-2)	2
c4.0.14: four-oligo latch seequences for bottom lid	box 4_C: row A (3-4)	2
c4.0.15: top lid oligos @ latches -latches	box 4_C: row A (5-6)	2
c4.0.16: bottom lid oligos @ latches -latches	box 4_C: row A (7-8)	2
c4.0.17: two-oligo latch barrel displacement oligos	box 4_C: row A (9-12)	4
c4.0.18: two-oligo latch top lid displacement oligos	box 4_C: row B (1-2)	2
c4.0.19: two-oligo latch bottom lid displacement oligos	box 4_C: row B (3-4)	2
c4.0.20: four-oligo latch barrel displacement oligos	box 4_C: row B (5-8)	4
c4.0.21: four-oligo latch top lid displacement oligos	box 4_C: row B (9-10)	2
c4.0.22: four-oligo latch bottom lid displacement oligos	box 4_C: row B (11-12)	2
c4.0.23: barrel zipper oligos	box 4_C: row C (1-4)	4
c4.0.24: top lid zipper oligos	box 4_C: row C (5-6)	2
c4.0.25: bottom lid zipper oligos	box 4_C: row C (7-8)	2

Working Stocks	Description	Pre-Working Stocks	Water	Total
c4.0A	no latches, no aptamers	1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL), 10 (4 μL), 15 (2 μL), 16 (2 μL)	39 μL	200 μL
c4.0B.core	latch design 1, no aptamers	1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL)	47 μL	200 μL
c4.0B.latches	latch design 1, no aptamers	8 (4 μL), 11 (2 μL), 12 (2 μL)	-	-
c4.0C.core	latch design 2, no aptamers	1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL)	47 μL	200 μL
c4.0C.latches	latch design 2, no aptamers	9 (4 μL), 13 (2 μL), 14 (2 μL), 23 (4 μL), 24 (2 μL), 25 (2 μL)	-	-

Protocol

   - mixed 10 μL of each 50 μM stock oligo to make pre-working stocks
   - mixed listed pre-working stocks to make working stocks where final concentration of each oligo is 250 nM