Table of c4.0 Working Stocks

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Working Stocks with Aptamers

Stock IDExperiment12345678910111213141516171819202122232425
c4.0Ano latches, no aptamersxxxxxxxx
c4.0Blatch design 1, no aptamersxxxxxxxx
c4.0Clatch design 2, no aptamersxxxxxxxxxxx
c4.0Dno latches, inside aptamersxxxxxxxx
c4.0Eno latches, outside aptamersxxxxxxxx
c4.0Flatch design 1, inside aptamersxxxxxxxx
c4.0Glatch design 1, outside aptamersxxxxxxxx
c4.0Hlatch design 2, inside aptamersxxxxxxxxxxx
c4.0Ilatch design 2, outside aptamersxxxxxxxxxxx
c4.0Jdisplace latch design 1xxx
c4.0Kdisplace latch design 2xxx


Working Stocks for Biotinylated Oligos

Stock IDExperiment123456789101112131415161718192021222324254b6b
c4.0Fblatch design 1, inside biotinxxxxxxxx
c4.0Gblatch design 1, outside biotinxxxxxxxx
c4.0Hblatch design 2, inside biotinxxxxxxxxxxx
c4.0Iblatch design 2, outside biotinxxxxxxxxxxx


Preworking Stocks

Description Plate Locations Total
c4.0.1: core barrel oligosbox 4_A: row A (1-12), row B (1-12), row C (1-12), row D (1-12), row E (1-12), row F (1-6)66
c4.0.2: core top lid oligosbox 4_A: row F (7-12), row G (1-12), row H (1-12); box 4_B: row A (1-3)33
c4.0.3: core bottom lid oligosbox 4_B: row A (4-12), row B (1-12), row C (1-12)33
c4.0.4: barrel oligos @ outside aptamers -aptamersbox 4_B: row D (1-4)4
c4.0.5: barrel oligos @ outside aptamers +aptamersbox 4_B: row D (5-8)4
c4.0.6: barrel oligos @ inside aptamers -aptamersbox 4_B: row D (9-12)4
c4.0.7: barrel oligos @ inside aptamers +aptamersbox 4_B: row E (1-4)4
c4.0.8: two-oligo latch sequences for barrelbox 4_B: row E (5-8)4
c4.0.9: four-oligo latch sequences for barrelbox 4_B: row E (9-12)4
c4.0.10: barrel oligos @ latches -latchesbox 4_B: row F (1-4)4
c4.0.11: two-oligo latch sequences for top lidbox 4_B: row F (5-6)2
c4.0.12: two-oligo latch sequences for bottom lidbox 4_B: row F (7-8)2
c4.0.13: four-oligo latch sequences for top lidbox 4_C: row A (1-2)2
c4.0.14: four-oligo latch seequences for bottom lidbox 4_C: row A (3-4)2
c4.0.15: top lid oligos @ latches -latchesbox 4_C: row A (5-6)2
c4.0.16: bottom lid oligos @ latches -latchesbox 4_C: row A (7-8)2
c4.0.17: two-oligo latch barrel displacement oligosbox 4_C: row A (9-12)4
c4.0.18: two-oligo latch top lid displacement oligosbox 4_C: row B (1-2)2
c4.0.19: two-oligo latch bottom lid displacement oligosbox 4_C: row B (3-4)2
c4.0.20: four-oligo latch barrel displacement oligosbox 4_C: row B (5-8)4
c4.0.21: four-oligo latch top lid displacement oligosbox 4_C: row B (9-10)2
c4.0.22: four-oligo latch bottom lid displacement oligosbox 4_C: row B (11-12)2
c4.0.23: barrel zipper oligosbox 4_C: row C (1-4)4
c4.0.24: top lid zipper oligosbox 4_C: row C (5-6)2
c4.0.25: bottom lid zipper oligosbox 4_C: row C (7-8)2


Working Stocks Description Pre-Working Stocks Water Total
c4.0Ano latches, no aptamers1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL), 10 (4 μL), 15 (2 μL), 16 (2 μL)39 μL200 μL
c4.0B.corelatch design 1, no aptamers1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL)47 μL200 μL
c4.0B.latcheslatch design 1, no aptamers8 (4 μL), 11 (2 μL), 12 (2 μL)--
c4.0C.corelatch design 2, no aptamers1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL)47 μL200 μL
c4.0C.latcheslatch design 2, no aptamers9 (4 μL), 13 (2 μL), 14 (2 μL), 23 (4 μL), 24 (2 μL), 25 (2 μL)--


Protocol

   - mixed 10 μL of each 50 μM stock oligo to make pre-working stocks
   - mixed listed pre-working stocks to make working stocks where final concentration of each oligo is 250 nM
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