Talk:20.109(F09): Mod 1 Day 8 FACS analysis

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Sign up for time on the FACS. Please arrive at the time you choose and then expect to spend 45 minutes in TC and 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.

Contents

T/R FACS schedule

team color FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM)
Red II
Orange
Yellow
Green II
Blue I
Pink I
Purple I
Silver


T/R FACS Data

Frequency of Parent for Green Cells

Sample Red Orange Green Blue Pink Purple Silver
Mock 0 xx xx xx xx xx xx
EGFP 5.82 xx xx xx xx xx xx
D5 0 xx xx xx xx xx xx
D3 0 xx xx xx xx xx xx
D5:D3 1:1 0.25 xx xx xx xx xx xx
D5:D3 1:0.5 0.061 xx xx xx xx xx xx
D5:D3 1:2 0.13 xx xx xx xx xx xx
D5:D3 1:10 0.017 xx xx xx xx xx xx



T/R lab

Below are links to the flow data from today.

Red Orange Yellow Green
Blue Purple Pink

I pulled all the numbers from each image and put them on one jpg. You'll have to zoom in and scroll to see the numbers that you want. All of the graphs are combined into one image here if it is easier for you to compare data that way.

Don't forget to average a few groups together. Yellow and Orange, you were suppose to average yours together, but neither of you got 'ideal' data, so each of you average yours with two others that had controls like we expected.

In the first 3 groups from today, remember that we only did Pink's D3 and D5 control, and we assumed that Blue and Purple worked (not a good idea normally, but we were crunched for time). Everyone think about (and write about) why it was important to include the D3 and the D5 controls.

The percentage of parent for the green cells is the percent of live cells that is green. This is the important number!

W/F FACS schedule

team color FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM)
Red I
Orange
Yellow
Green III
Blue Time block II
Pink II
Purple II
Silver

W/F Lab

Don't forget to average a few groups together. Everyone think about (and write about) why it was important to include the D3 and the D5 controls. The percentage of parent for the green cells is the percent of live cells that is green. This is the important number!

Red Green Blue Purple
Pink All WF Stats All WF Images
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