Talk:20.109(F10): Mod 2 Day 7 Readout of Protein

From OpenWetWare

Jump to: navigation, search

Western Blot Results

All team blots shown below (except red which chose not to develop theirs).
Each team will need to label their lanes.
Team Pink did a second Western with interesting results. They should add labels and a brief explanation of their work.

Blue Team
Blue Team
Green Team
Green Team
Pink Team
Pink Team
Orange Team
Orange Team
Silver Team
Silver Team
Yellow Team
Yellow Team
Grape Team
Grape Team
Pink^2 Team
Pink^2 Team


beta-gal Activity assay (Blue team)

Error bars denote 1 stdev from the mean. Experiments were performed in triplicate.
Mut1 has an MGG mutation
Mut2 is unsequenced, but is probably loss-of-function
Also, L and D were mistakenly swapped (L bars denote dark trials, D bars denote light trials)
Image:Graph1.png


Western Blot Pink^2 (Shown Above)

Comparing Cph8 expression between NB334, NB188+pCph8, Mutant Candidate 1, Mutant Candidate 2
Also comparing Cph8 expression between light and dark exposed cultures for each

From right to left
1 Kal Marker
2 H6EnvZ
3 NB334 Light
4 NB334 Dark
5 NB188+pCph8 Light
6 NB188+pCph8 Dark
7 Mutant 1 Light
8 Mutant 1 Dark
9 Matant 2 Light
10 Mutant 2 Dark

Personal tools