Talk:20.109(F10): Mod 2 Day 7 Readout of Protein
Western Blot Results
All team blots shown below (except red which chose not to develop theirs).
Each team will need to label their lanes.
Team Pink did a second Western with interesting results. They should add labels and a brief explanation of their work.
beta-gal Activity assay (Blue team)
Error bars denote 1 stdev from the mean. Experiments were performed in triplicate.
Mut1 has an MGG mutation
Mut2 is unsequenced, but is probably loss-of-function
Also, L and D were mistakenly swapped (L bars denote dark trials, D bars denote light trials)
Western Blot Pink^2 (Shown Above)
Comparing Cph8 expression between NB334, NB188+pCph8, Mutant Candidate 1, Mutant Candidate 2
Also comparing Cph8 expression between light and dark exposed cultures for each
From right to left
1 Kal Marker
2 H6EnvZ
3 NB334 Light
4 NB334 Dark
5 NB188+pCph8 Light
6 NB188+pCph8 Dark
7 Mutant 1 Light
8 Mutant 1 Dark
9 Matant 2 Light
10 Mutant 2 Dark
