Talk:20.109(S09):Start-up protein engineering (Day1)
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TR lab
Team colour | Mutation X#Z | Primer (forward) | Silent mutation | New restriction site |
Teaching sample | S101L | GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G | Ala 103 | FspI |
Red | D131G | G ATA AGG GAA GCA GAT ATC GGT GGT GAT GGC CAA GTT AAC TAT | Val 136 | HpaI |
Orange | E31I | C GCG CGC ATC ACC ACA AAG ATA CTG GGC ACC GTT ATG Ordered: GC ACC ATC ACC ACA AAG ATA CTG GGC ACC GTT ATG AGG |
Leu 32 | XcmI |
Yellow | D133R | AGG GAA GCA GAT ATC GAT GGT CGT GGC CAA GTA AAC TAT GAA GAG Ordered: AGG GAA GCA GAT ATC GAT GGT CGT GGC CAA GTT AAC TAT GAA GAG |
Val 136 | HpaI |
Green | D133W | GG GAA GCA GAT ATC GAT GGT TGG GGC CAA GTT AAC TAT G | Val 414 | HpaI |
Blue | D58H | GAA GTC GAT GCG CAT GGC AAT GGA ACG ATT TAC | Ala 57 | FspI |
Pink | M144D | GAA GAG TTC GTA CAA GAC ATG ACA GCA AAG TAA GAA | Phe 419, Glu 418 | EcoRI |
Purple | D93P | CGA GAA GCT TTC CGT GTT TTT CCC AAG GAT GGG AAC GGC | Ala 88 | HindIII |
Grey | D22W | GCC TTC TCA TTA TTC GAC AAG TGG GGG GAC GGC AAC ATC ACC Ordered: GCC TTC TCA TTA TTC GAC AAG TGG GGA GAC GGC AAC ATC ACC |
Gly 301 | BsmBI |
WF lab
Team colour | Mutation X#Z | Primer (forward) | Silent mutation | New restriction site |
Teaching sample | S101L | GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G | Ala 103 | FspI |
Red | D93H actual: D95H |
GCA TTC CGT GTT TTT GAC AAG CAT GGG AAC GGA TAC ATC AGT GC | Gly 379 Ser 388 | BaeI |
Orange | E127K | GTT GAT GAA ATG ATC AGG AAA GCA GAT ATC GAT GGT GAT GGC | Ile 403 | BclI |
Yellow | L105S | C AGC GCT GCT CAG TCA CGT CAC GAC ATG ACA AAC Ordered: C AGC GCT GCT CAG TCA CGA CAC GTC ATG ACA AAC |
Arg384 | AflIII |
Green | T79P | GA AAA ATG AAG GAC CCA GAC AGC GAA GAG G Ordered: see Green/Purple |
Thr 79 | PpuMI |
Blue | E31R | ACC ATC ACC ACA AAG AGG CTG GGC ACC GTT ATG AGG | Leu 310 | BseYI |
Pink | M124S | GAA GTT GAT GAA TCG ATA AGG GAA GCA GAT ATC GAT GG | none | ClaI |
Purple | T79P | GA AAA ATG AAG GAC CCA GAC AGC GAA GAG GAA ATC CGA GAA GC Ordered: see Green/Purple |
PpuMI | |
Grey | L32D | GGC ACC ATC ACC ACA AAG GAA GAT GGT ACC GTT ATG AGG | KpnI | |
Green/Purple | T79P | GA AAA ATG AAG GAC CCA GAC AGC GAA GAG GAA ATC C | Thr 79 | PpuMI |