Talk:Eccles:DNA Isolation

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Air-drying

Is the following part of the actual protocol, or a typo?

"5. Spin 5 min, carefully decant supernatant and air dry pellets (upside-down, 10-30 min at room temp). Wash pellet with 600ul room temp 70% ethanol. Spin again 5 min.

6. Carefully decant supernatant and air dry pellets (upside-down, 10-30 min at room temp). "

You don't need to air-dry before the 70% EtOH wash - remove supernatant, wash, remove supernatant, dry is the conventional method, Ajeffs 22:43, 30 July 2006 (EDT).

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