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Welcome to your project page!
For each part listed, you should:
- Design oligos to make your part
- Write up proper construction files and put it on the Construction Files page
- Put your oligos on the Oligo Log page
- Put your part sequence on the part document
- Name your parts according to the list here
EhaB Autotransporter
Source: E. coli strain O157:H7 genomic DNA
You are going to construct a display part derved from the ehaB gene. The sequence is at NC_002655 in pubmed. You should include amino acids 573-980 (through to the stop codon). For background, you should read PMID: 18237301.
This part encodes a N-terminal display protein
Your displayer part should be of the {<part!} style (no start, with stop). There should be NO prepro sequence in your part. You should design your construction file to insert your part into plasmid pBca9495KC-Bca1144#5 using EcoRI and BamHI. The map of this plasmid is here.
Leucine Zipper peptide EILD
Source: Synthetic
You should read PMID: 12459719. You will make a BglBrick part encoding:
VKELEDKNEELLSeiYHldNEVARLKKLVGERGGC
No start, no stop, in frame.
This part encodes a passenger protein
Your passenger part should be of the {<part>} style (no start, no stop). There should be NO prepro sequence in your part. If your part is naturally secreted, you should encode only the active peptide. You should design your construction file to insert your part into plasmid pBca9495AK-Bca1144#5 using EcoRI and BamHI. The map of this plasmid is here.
