Template:SBB10 ConstructionFiles CarolynK sbb24
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Construction of O99 5'UTR Basic Part sbb24
PCR sbb24F and sbb24R on pEC52 (468 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144 (EcoRI/BamHI, 2472+918, L)
Product is pBca9523-sbb24 {O99 5'UTR}
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sbb24F Forward oligo for cloning of sbb24 ccataGAATTCatgAGATCTacgattctgacgcattttttatg
sbb24R Reverse oligo for cloning of sbb24 ctgatGGATCCacgtcaaaaaccagccagaactgcg
JCA Notes
- Vector sizes slightly off, but otherwise correct
PCR sbb24F and sbb24R on pEC52 (468 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144 (EcoRI/BamHI, 2472+910, L) Product is pBca9523-sbb24 {O99 5'UTR} ------------------------------------- sbb24F Forward oligo for cloning of sbb24 ccataGAATTCatgAGATCTacgattctgacgcattttttatg sbb24R Reverse oligo for cloning of sbb24 ctgatGGATCCacgtcaaaaaccagccagaactgcg
