TempliPhi

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Contents

Overview

A kit for in vitro amplification of plasmid DNA for sequencing. It enables sequencing direct from a single colony thereby avoiding overnight cultures and minipreps. It is also useful for sequencing low copy plasmids which give poor yields from minipreps. This kit is based on the φ29 DNA polymerase and uses rolling circle amplification.

Materials

Specific protocols

References

Error fetching PMID 11381035:
Error fetching PMID 14676313:
  1. Error fetching PMID 11381035: [Dean-GenomeRes-2001]
  2. Error fetching PMID 14676313: [Reagin-JBiomolTech-2003]
  3. TempliPhi FAQ

    [FAQ]

All Medline abstracts: PubMed HubMed
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