User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/09

Alien genes

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Alien Gene New Primer Screen Reaction mixture conc. 1x 12 Template CCA3 1ng/ul 1 12 TopTaq buffer 10x 1 12 dNTP mix 10 mM each 0.2 2.4 Primer mix 5 uM each 0.5 TopTaq (QIAGEN) 5U/ul 0.05 0.6 Milli Q water 7.25 87 10 ul PCR cycle 94C 3 min 98C 10 sec 60C 3 min x35 72C 5 min Load 5.0ul sample AMP Lane Contents Fosmid 1 AV10081 AV_TEL_L_A 2 AV10086 AV_TEL_L_A 3 AV10091 AV_TEL_L_A 4 AV10092.2 AV_TEL_L_A 5 AV10093.1 AV_TEL_L_A 6 AV10093.2 AV_TEL_L_A 7 AV100100 AV_TEL_L_A 8 AV10106 AV_TEL_L_B 9 AV10108 AV_TEL_L_B 10 AV10111 AV_TEL_L_B 11 POS Control AV10008