Tasks for February 20
- Bacteria studies
- To prepare t-plates for adhesive strength test
Bacteria studies
Want to test whether TiO2 content in films has any effect on bacteria populations in the dark and whether it inhibits their growth.
- Prepare Agar Plates
- Prepare Saline Solution
Bacterial Inactivation Evaluation in the Dark
- The tubes containing the bacteria and broth were cloudy, confirming the growth of the bacteria
- Clean a TiO2/Epoxy film and an Epoxy film with ethanol to kill all bacteria
- Wash the TiO2/Epoxy film a few times to clean any TiO2 from the surface
- Clean lab tweezers with ethanol as well.
- Prepare two petri dishes for the films
- Make sure the film are dried out of the ethanol before placing them in the petri dish
- Vortex the broth/bacteria tubes
- Pipette 600 micro liter of the bacteria/broth solution onto the film
- Try to even out the surface as much as you can
- Cover the petri dish so no evaporation occurs
- Place in drawer
Recipe for Agar Plates
- Add 3g of tryptone, 1.5 g of yeast, 3 g of NaCl, and 4.5 g of Agar to 300 mL of water
- Autoclave
Saline Solution Recipe
- 12.5 g of NaCl in 500 mL of water
- Autoclave
Preparation of metal t-plates for adhesive strength test
- a smaller batch was made given the size of the t-plates (Half the usual amount)
- After mixing, the liquid coating was spread onto the bottom piece of metal and covered with the top piece of metal
- Metal pieces were left to stand until next class to be cured.
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