User:Anthony Salvagno/Notebook/Research/2009/11/17/Ligating New Constructs

Setup 1

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• 30' - added 3ul more
• 60' - added 3ul more
• 90' - added 3ul more

Setup 2

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I kinda messed up in that I added 2.5ul of Adapter insead of the 2ul I had planned. This isn't a huge deal since it allows me to put in more than what is needed.

• 30' - added 2.5ul more
• 60' - added 2.5ul more
• 90' - added 2.5ul more

Next Time

Tomorrow I will run a gel of the results and pray to the biology gods that there is successful ligation.