User:Anthony Salvagno/Notebook/Research/2010/11/04/More Bead Profiles

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Today we did some DOG profiles, but we put some foam under the slide holder to see if it would help damping. I also ordered a new batch of 0.51um beads from Bangs. I will use these new beads to do tests with the small beads and to work on tethering.

Results

<html><div style="width:425px" id="__ss_5670221"><strong style="display:block;margin:12px 0 4px"><a href="http://www.slideshare.net/anthonysalvagno/dog-scanswithfoam" title="Dog scans-with-foam">Dog scans-with-foam</a></strong><object id="__sse5670221" width="425" height="355"><param name="movie" value="http://static.slidesharecdn.com/swf/ssplayer2.swf?doc=dog-scans-with-foam-101104142013-phpapp01&rel=0&stripped_title=dog-scanswithfoam&userName=anthonysalvagno" /><param name="allowFullScreen" value="true"/><param name="allowScriptAccess" value="always"/><embed name="__sse5670221" src="http://static.slidesharecdn.com/swf/ssplayer2.swf?doc=dog-scans-with-foam-101104142013-phpapp01&rel=0&stripped_title=dog-scanswithfoam&userName=anthonysalvagno" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="425" height="355"></embed></object><div style="padding:5px 0 12px">View more <a href="http://www.slideshare.net/">presentations</a> from <a href="http://www.slideshare.net/anthonysalvagno">Anthony Salvagno</a>.</div></div></html>

For the first couple of scans I wanted to reenact the experiments I did yesterday. I wanted to test to see if rapidly scanning a bead would affect the noise in the data. The reason I think this is because the noise from yesterday was very periodic (explained by an oscillation ie the stage moving up and down) and I got one data set that had very little noise.

The first two scans are done in rapid succession. I would scan a bead and as soon as the trap passed over the bead I would release the button and press it again to start the next scan. I did this 4 times in a row and analyzed the data of the last two plots (shown in the slides above). I did look at the other results and they were similar to those shown above.

For the third scan I gave about 30 seconds between scans to give the stage time to relax and then scanned. The result is way less noise. I did this several times as well and all the results looked similar.

The final scan shown needs some explanation. We did the first three scans shown with some foam wedged between the breadboard on the microscope and the sample holder. The final scan was done with a relaxed stage and NO foam. The result is a slightly more noisy data set.

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