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Registration/Questionnaire: 20.109 Fall 2007
Major: 20 Minor: 14&15
Year of Graduation
ANaskar AT mit DOT edu
Have you taken
7.05/5.07 (Biochemistry) yes
7.06 (Cell Biology) no
7.02 (General Biology Lab) no
5.310 (General Chemistry Lab) no
Do you have any experience culturing cells (mammalian, yeast or microbial)?
Do you have any experience in molecular biology (electrophoresis, PCR, etc)?
Please briefly describe any previous laboratory experience
I have worked in a UROP setting since fall of 2006. I am familiar with basic laboratory equipment and safety but have never dealt with biology-related lab work
Anything else you would like us to know?
Research Proposal Link
M13 Re-Engineering Ideas
|I||Assembly||control channel/secretion to make it more selective|
|II||Replication of DNA + strand||make it so replication is faster and more efficient|
|III||Phage tail protein (5 copies)||make it so it has a more diverse contact site, not only TolA|
|V||Binds ssDNA||increase amount of p5 to see how the rate of replication changes|
|VI||Phage tail protein (5 copies)||more copies of p6 to see if rate of infection is altered|
|VII||Phage head protein (5 copies)||attach GFP so we can know when phages are being secreted|
|VIII||Phage coat protein (2700 copies)||alter p8 to bind w/ other proteins, not only E.Coli|
|IX||Phage head protein (5 copies)||attach GFP|