User:Beatriz Gimenez De C./Notebook/571/2014/11/11

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Tasks New lysozyme calibration in the range 20-100 μL To run dialysis of 0.6 g/L lysozyme vs 5-45 mM CaCl2 using MWCO 3500 Creation of bradford calibration curve (Using 0.6 g/L lysozyme stock) Lysozyme calibration curve at 578 nm Ca2+ ISE CaCl2 concentration (mM) Potential measurement (mV) 5 (1) 59.1 15 (2) 70.5 25 (3) 72.2 35 (4) 75.9 45 (5) 79.1 Substance Potential measurement (mV) Lysozyme 1 53.9 Lysozyme 2 65.6 Colloid 3 71.8 Colloid 4 78.6 Colloid 5 82.7