Objective
The objective for this experiment is to prepare gold and silver nanoparticles using nutritional soy protein isolate, micellar casein, whey protein isolate, pea protein isolate and brown rice protein isolate
Materials
Soy Protein Isolate (NutraBio) - http://www.nutrabio.com/category/spi/
Whey Protein Isolate (NutraBio) - http://www.nutrabio.com/product/WPI2/
Micellar Casein (NutraBio) - http://www.nutrabio.com/product/CAS2/
Pea Protein Isolate (Swanson Health Products) http://www.swansonvitamins.com/swanson-organic-certified-organic-pea-protein-powder-non-gmo-1-lb-454-grams-pwdr#label
Sprouted Brown Rice Protein (Naked Nutrition) - http://nkdnutrition.com/products/organic-brown-rice-protein-powder
Protein concentrations are detailed on reaction setup. Concentrations were determined accurately through changing volume for a particular mass.
Procedure
This is the first day testing these nutritional proteins, the procedure from 01/27/16 is copied with these proteins as a starting point.
Reaction Setup Detailed below
| '
|
'
|
2.5mM HAuCl4 (µL)
|
2.5mMAgNO3 (µL)
|
Protein (µL)
|
2.5mM Fructose (µL)
|
Water (µL)
|
Au/Ag:Protein
|
Notes
|
|
1 |
40 |
0 |
560 |
0 |
400 |
1:15 |
|
|
2 |
40 |
0 |
440 |
0 |
520 |
1:11 |
|
|
3 |
40 |
0 |
360 |
0 |
600 |
1:09 |
|
|
4 |
40 |
0 |
280 |
0 |
680 |
1:07 |
|
|
5 |
40 |
0 |
200 |
0 |
760 |
1:05 |
|
|
6 |
0 |
40 |
560 |
0 |
400 |
1:15 |
|
|
7 |
0 |
40 |
360 |
0 |
600 |
1:09 |
|
|
8 |
0 |
40 |
200 |
0 |
760 |
1:07 |
|
|
9 |
40 |
0 |
560 |
0 |
400 |
1:15 |
Formed white fibrous bundles 9-11 (precipitate?)
|
|
10 |
40 |
0 |
440 |
0 |
520 |
1:11 |
|
|
11 |
40 |
0 |
360 |
0 |
600 |
1:09 |
|
|
12 |
40 |
0 |
280 |
0 |
680 |
1:07 |
purple nanofibers
|
|
13 |
40 |
0 |
200 |
0 |
760 |
1:05 |
purple nanofibers
|
|
14 |
0 |
40 |
560 |
0 |
600 |
1:15 |
14-22slighty cloudy, white accumulation at bottom (protein precipitate?)
|
|
15 |
0 |
40 |
560 |
20 |
580 |
1:15 |
|
|
16 |
0 |
40 |
560 |
40 |
560 |
1:15 |
|
|
17 |
0 |
40 |
360 |
0 |
600 |
1:09 |
|
|
18 |
0 |
40 |
360 |
20 |
580 |
1:09 |
|
|
19 |
0 |
40 |
360 |
40 |
560 |
1:09 |
|
|
20 |
0 |
40 |
200 |
0 |
600 |
1:07 |
|
|
21 |
0 |
40 |
200 |
20 |
580 |
1:07 |
|
|
22 |
0 |
40 |
200 |
40 |
560 |
1:07 |
|
|
23 |
40 |
0 |
560 |
0 |
400 |
1:15 |
*All whey samples have double volume of protein
|
|
24 |
40 |
0 |
440 |
0 |
520 |
1:11 |
23 (white precipitate at bottom)
|
|
25 |
40 |
0 |
360 |
0 |
600 |
1:09 |
(24-27) white stringy fibers climbing tube walls
|
|
26 |
40 |
0 |
280 |
0 |
680 |
1:07 |
|
|
27 |
40 |
0 |
200 |
0 |
760 |
1:05 |
|
|
28 |
0 |
40 |
560 |
0 |
600 |
1:15 |
28-30 clear
|
|
29 |
0 |
40 |
560 |
20 |
580 |
1:15 |
|
|
30 |
0 |
40 |
560 |
40 |
560 |
1:15 |
|
|
31 |
0 |
40 |
360 |
0 |
600 |
1:09 |
31-33 clear
|
|
32 |
0 |
40 |
360 |
20 |
580 |
1:09 |
|
|
33 |
0 |
40 |
360 |
40 |
560 |
1:09 |
|
|
34 |
0 |
40 |
200 |
0 |
600 |
1:07 |
34-36 clear
|
|
35 |
0 |
40 |
200 |
20 |
580 |
1:07 |
|
|
36 |
0 |
40 |
200 |
40 |
560 |
1:07 |
|
|
37 |
40 |
0 |
560 |
0 |
400 |
1:15 |
37-40 purple and white fibers
|
|
38 |
40 |
0 |
440 |
0 |
520 |
1:11 |
|
|
39 |
40 |
0 |
360 |
0 |
600 |
1:09 |
|
|
40 |
40 |
0 |
280 |
0 |
680 |
1:07 |
|
|
41 |
40 |
0 |
200 |
0 |
760 |
1:05 |
41 only purple fibers
|
|
42 |
0 |
40 |
560 |
0 |
600 |
1:15 |
42-44 white precipitate at bottom
|
|
43 |
0 |
40 |
560 |
20 |
580 |
1:15 |
|
|
44 |
0 |
40 |
560 |
40 |
560 |
1:15 |
|
|
45 |
0 |
40 |
360 |
0 |
600 |
1:09 |
45-47 white precipitate at bottom
|
|
46 |
0 |
40 |
360 |
20 |
580 |
1:09 |
|
|
47 |
0 |
40 |
360 |
40 |
560 |
1:09 |
|
|
48 |
0 |
40 |
200 |
0 |
600 |
1:07 |
48-50 white precipitate at bottom
|
|
49 |
0 |
40 |
200 |
20 |
580 |
1:07 |
|
|
50 |
0 |
40 |
200 |
40 |
560 |
1:07 |
|
|
51 |
40 |
0 |
560 |
0 |
400 |
1:15 |
51-55 white precipitate with purple fibers near bottom of tube
|
|
52 |
40 |
0 |
440 |
0 |
520 |
1:11 |
|
|
53 |
40 |
0 |
360 |
0 |
600 |
1:09 |
|
|
54 |
40 |
0 |
280 |
0 |
680 |
1:07 |
|
|
55 |
40 |
0 |
200 |
0 |
760 |
1:05 |
|
|
56 |
0 |
40 |
560 |
0 |
600 |
1:15 |
56-58 white precipitate at bottom of tube and white particles (dots, fibers) clinging to walls
|
|
57 |
0 |
40 |
560 |
20 |
580 |
1:15 |
|
|
58 |
0 |
40 |
560 |
40 |
560 |
1:15 |
|
|
59 |
0 |
40 |
360 |
0 |
600 |
1:09 |
59 - 60 white precipitate at bottom of tube and white particles (dots, fibers) clinging to walls
|
|
60 |
0 |
40 |
360 |
20 |
580 |
1:09 |
|
|
61 |
0 |
40 |
360 |
40 |
560 |
1:09 |
61 tan precipitate at bottom of tube, no fibers clinging to the sides
|
|
62 |
0 |
40 |
200 |
0 |
600 |
1:07 |
59 - 60 white/yellow/black precipitate at bottom of tube and white particles (dots, fibers) clinging to walls
|
|
63 |
0 |
40 |
200 |
20 |
580 |
1:07 |
|
|
64 |
0 |
40 |
200 |
40 |
560 |
1:07 |
|
| Whey
|
2.5mM HAuCl4 (µL)
|
2.5mMAgNO3 (µL)
|
Protein (µL)
|
2.5mM Fructose (µL)
|
Water (µL)
|
Notes
|
| 23A |
40 |
0 |
560 |
0 |
400 |
23A white fibers
|
| 24A |
40 |
0 |
440 |
0 |
520 |
24A white fibers
|
| 25A |
40 |
0 |
360 |
0 |
600 |
25A purple nanoparticles with dark pellet at bottom
|
| 26A |
40 |
0 |
280 |
0 |
680 |
26A light purple (white and purple) nanofibers at bottom
|
| 27A |
40 |
0 |
200 |
0 |
760 |
27A slightly pink solution nanoparticles
|
| 28A |
0 |
40 |
560 |
0 |
600 |
28-33 clear
|
| 29A |
0 |
40 |
560 |
20 |
580 |
|
| 30A |
0 |
40 |
560 |
40 |
560 |
|
| 31A |
0 |
40 |
360 |
0 |
600 |
|
| 32A |
0 |
40 |
360 |
20 |
580 |
|
| 33A |
0 |
40 |
360 |
40 |
560 |
|
| 34A |
0 |
40 |
200 |
0 |
600 |
34A, cloudy white fibers at bottom of tube
|
| 35A |
0 |
40 |
200 |
20 |
580 |
clear
|
| 36A |
0 |
40 |
200 |
40 |
560 |
clear
|
Results
Graphs for particular gold and silver NP species by protein are shown below
- One new color type indicative of AuNP was made, purple Whey AuNP Solution, however, when UV vis spectra was taken, noting appeared. Its possible that protein did not dissolve fully and thus only very few NPs were made
Figure 1: UVVIS Spectra for CaseinAgNP Synthesized 02/02/15
Note CaseinAg NP for this reaction time (4 hours 80˚C) peak at 420nm
|
Project name
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