User:Cassandra M Barrett/Notebook/Open Chromatin/2015/09/26
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LCR Transformations and gel results
Purpose: to test effectiveness of LCR by transforming E. coli with ligated DNA
Follow standard DH5alpha transformation protocol:
Run 2uL of reactions 1-11 on gel
We see vague larger bands on 10 and 11, probably just reannealed MV10 No sample in rxn 8 tube for some mysterious reason....