User:Daniel B Rosen/Notebook/Methylation Sensor/2011/10/12

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Daniel B Rosen 20:48, 12 October 2011 (EDT)

Finish Maxi Prep ppGFP plasmids (2,4,6,8)

Blank TE Sample 2, reran

Sample ID User name Date and Time Nucleic Acid Conc. Unit A260 A280 260/280 260/230 Sample Type Factor
2 Rosen, Daniel B./Sloan-Kettering Institute 10/12/2011 8:56:47 PM -975.3 ng/µl -19.506 -10.145 1.92 2.25 DNA 50
4 Rosen, Daniel B./Sloan-Kettering Institute 10/12/2011 8:57:49 PM 3572.5 ng/µl 71.45 37.114 1.93 2.3 DNA 50
6 Rosen, Daniel B./Sloan-Kettering Institute 10/12/2011 8:58:25 PM 4108.9 ng/µl 82.178 42.875 1.92 2.28 DNA 50
8 Rosen, Daniel B./Sloan-Kettering Institute 10/12/2011 8:58:54 PM 3767.8 ng/µl 75.356 39.215 1.92 2.28 DNA 50
2 Rosen, Daniel B./Sloan-Kettering Institute 10/12/2011 8:59:32 PM 3699.2 ng/µl 73.983 38.488 1.92 2.3 DNA 50

In Silico Build of mCherry plasmids (9,10,11,12)

Digest Confirmation of mCherry Plasmids

Passage 293T cells (1x15 cm --> 2x; 1x 15 cm --> 2x6well)

note: 2 6 wells prepared with collagenase yesterday 6.94E6 cells/mL seeded at 2mL/well in 6 well plates (2x) 5.85E6 cells/mL seeded at 2.825E6 cells/mL at 25mL/plate in 15 cm dish (2x)


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