User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/06/01

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The Daily Grind

  • Describe colony morphology of the new microbes spotted on R2A
  • Mix and ligate 16S fragments from all seeds for cloning (then do the ligation overnight)
  • Cut up the unbacterialized potatoes for propagation
  • Put 10 colonies of ACC deaminase in either pSB1A2 or pDSK-GFPuv in either 1 mL of LGI or 1 mL of LGI plus 100 mM ACC. Include plasmid free E. coli as negative control.

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