User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/01/06

Applied Soil Microbial Ecology

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Remains of the Day Redo qPCR of PR1 in potato and tomato Call Norgen about tubes in soil DNA extraction kits (they are gonna look into giving us new ones) Talk to George about PCR results and agree about TRFLP method and agreement meeting Look at data and see about explaining outlier in Brazil trial New bacterial primers use a specific recipe (I find these concentrations/volumes/times weird so discuss): dH2O - 37.5 ul 10X Buffer - 5 ul MgCl2 (50 mM) - 2 ul BSA (20 mg/mL) - 1.25 ul dNTPs (4 mM?) - 1 ul Primer 63F-Max550 (20 mM) - 1 ul Primer 1389-6FAM (20 mM) - 1 ul Taq Polymerase - 0.25 ul Total - 50 ul PCR program is 2 mins 95, 30 cycles (1 min 95, 1 min @60 degrees, 2 mins 72 degrees), 10 mins @ 72 dgrees