User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/10/15

Applied Soil Microbial Ecology

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Remains of the Day Work on addressing Manish's comments Transforming ligations from Friday with Steph Waiting for results from Guelph to write up potato and organic amendment reports Work on handout for meeting on Friday Figure out RT PCR primers for strawberry experiment B. subtilis primers will be from here: http://apsjournals.apsnet.org.proxy1.lib.uwo.ca/doi/abs/10.1094/PHYTO-06-12-0123-R P. fluorescens primers will be from here: http://aem.asm.org/content/73/17/5531.full.pdf+html B. phytofirmans primers will be from here (picking endophytic genes and designing primers against those cause no one has B. phytofirmans primers): http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639386/ Interstingly, I just discovered FB2 is Pseudomonas aeruginosa strain FB2 (ACCESSION: HQ658762) rather than P. fluorescens. ;-\