12/18/13
READING LIST - Assigned by Dr. Haynes
PROTOCOLS
- Bioinformatics workflow or finding PcTF targets: will be added this week after I work out some bugs
- Transfection of plasmid DNA into human cells: http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo
- RNA miniprep: http://openwetware.org/wiki/Haynes:TRIzol_RNeasy
- cDNA synthesis: Notebook page from Carly's project
- RT-PCR set-up: http://openwetware.org/wiki/Haynes:UPLassay (I fixed the assay design center link)
RESEARCH LITERATURE
Goal: be able to explain how these papers relate to your project...
- Wang YH, Wei KY, Smolke CD. (2013) Synthetic Biology: Advancing the Design of Diverse Genetic Systems. Annu Rev Chem Biomol Eng. 4:69-102 - Recent comprehensive review on the field of synthetic biology
- Haynes KA, Silver PA. (2009) Eukaryotic systems broaden the scope of synthetic biology. J Cell Biol. 187:589-96
- Haynes KA, Silver PA. (2011) Synthetic reversal of epigenetic silencing. J. Biol. Chem. 286:27176-82 - Pay particular attention to where the protocols listed above appear in this paper (and if they do)
- Bramswig NC, Everett LJ, Schug J, Dorrell C, Liu C, Luo Y, Streeter PR, Naji A, Grompe M, Kaestner KH. (2013) Epigenomic plasticity enables human pancreatic α to β cell reprogramming. J Clin Invest. 123:1275-84 - Pay particular attention to genes that are up-regulated after alpha cells are treated with the HDAC inhibitors. Make a list of these genes and enter the list into your OWW notebook
- Moss LG. (2013) Creating new β cells: cellular transmutation by genomic alchemy. J Clin Invest. 123:1007-10
Note: You can download these papers for free if you log in through http://www-ncbi-nlm-nih-gov.ezproxy1.lib.asu.edu/pubmed?otool=azuazslib
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