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Objective
Learn how to maintain an OpenWetWare Notebook.
Calculations
0.06229 g ÷ 898.65 g/mol RuComp × 1000 mmol/mol = 0.06932 mmol Ru Complex
1:1 Ru:ATP
0.06932 mmol ÷ 1000 mmol/mol × 507.18 g/mol = 0.0352g ATP
0.0352g ATP ÷ 507.18g/mol ATP × 0.596 mol DCC ÷ 0.197 mol ATP × 206.33g/mol DCC = 0.04332g DCC
Description
- The 0.0352g of ATP was dissolved in 0.1M TEAB and run in a column using 0.1M TEAB, collecting 5mL fractions.
- The fractions were spotted on a TLC plate and UV was used to determine which fractions contained ATP.
- Fractions 2, 3, and 4 contained ATP. These fractions were combined and rotovapped. The ATP was not visible after the rotovap.
- 10mL methanol was added and rotovapped (three times). (Once again, the ATP did not seem visible after the rotovap.)
- 1.8 mL DMF was added under Nitrogen
- A drop of DMF-ATP was spotted on a TLC plate, indicating the presence of ATP
- 0.04332 g DCC was added and the reaction ran for 3 hours.
- 0.06229g Ru Complex was dissolved in 10mL MeOH and added to ATP after the 3-hour reaction was completed.
- 0.25mL TEA was added to the mixture under Nitrogen and reacted for 30 minutes.
- 20mL of H2O was added (no longer under nitrogen) and the mixture was covered overnight.
- When the mixture was added to the water, a cloudy orange-yellow solution precipitates, which had not occurred before. Perhaps this was because of the 1:1 ratio.
- The solution was covered and left under the hood overnight.
Data
- Add data and results here...
Notes
This area is for any observations or conclusions that you would like to note.
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Phosphorylation Lab
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