User:Dominique Brager/Notebook/481 Notebook/2016/10/12

Biomaterials Design Lab

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Objective Continue running 20% column and 30% column. Pump is used on 30% with a speed of 50mL in 9:45 minutes. The pipette bulb is used on the 20% to speed it up. Because it has to be hand pumped and it's inconsistent, it's hard to determine a rate. Finished running UV-Vis on the 10% clay beads. Samples 31-34 were of 20mL. Description Ran data analysis on AC. Found the Max Absorbance for each run. Using the Beer's Law calibration Curve we found a few weeks ago (y=0.0813x) calculated concentration for each sample. Created plot of concentration vs. Volume Eluted. Calculated the amount of MG added to the Column 7 samples *5mL/sample*1L/1000mL*50mg/1L = 1.75mg Calculated the amount of MG eluted. From samples 1-7: 5mL*1L/1000mL*concentration of sample = mg of MG not adsorbed For sample 8: 1mL*1L/1000mL*concentration of sample = mg of MG not adsorbed 4mL*1L/1000mL*concentration of sample = mg of MG desorbed For samples 9-108: 5mL*1L/1000mL*concentration of sample = mg of MG desorbed Subtract mg of MG not adsorbed from amount of MG added to column to get amount of MG adsorbed by the column. Find capacity of beads by dividing mass of MG adsorbed by mass of beads Find capacity of activated carbon by dividing mass of MG adsorbed by mass of activated carbon in beads. Data Graph of Concentration vs. Volume Eluted for the Activated Carbon. Amount of MG loaded: 1.75mg Amount not adsorbed: 0.223mg Amount adsorbed: 1.75mg-0.223mg=1.527mg Amount desorbed: 0.992mg Mass of Beads in Column: 1.8075g Capacity of Beads: 0.8448 mg/g Mass of Activated Carbon in Column: 0.18075g Capacity of Activated Carbon: 8.448 mg/g Notes 10% clay data analysis started. Will be posted Friday. Mass of 20% column with beads was not taken before starting elution. Started column again.