User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/09
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High Stabilitiy Plasmid Development
P1004 Double Digestion
This digestion was already performed, yet I require more to isolate the P1004 part from a gel band. Two equal 70 μL reactions were prepared:
Band Isolation of P1004
A 1% low melting poit agarose gel was ran for 100min at 80V. Using the QIAgen kit protocol I obtained 40μL of the P1004 band. I will see it tomorrow in a gel.