User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/16

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High Stabilitiy Plasmid Development Abstract Dephosphatation of DD B0015 E,X in pSB1AK3 Ligation of repC-PCR and B0015 dephosphated Dephosphatation The reaction was incubated at 37°C and inactivated for 5 minutes at 65°C. Reactive Volume DD E,X B0015 in pSB1AK3 stock 1 17μL Antarctic Phosphatase 1μL Reaction Buffer 10x 2μL Total 20μL Ligation Reactive Volume DD E,S repC 4μL DD E,X B0015 in pSB1AK3 dephosph. 3μL T4 DNA ligase 0.5μL 5x buffer 4μL H2O mQ 8.5μL Total 20μL