User:Eric M. Walters/Notebook/Spring 2012/2012/04/11

Cyanobacterial acrylate production	Main project page Previous entry      Next entry
Enrichment of the real construct Gel extracted the ~5.5kb band out of a gel run of the whole 50 μL ligation Final conc: 6 ng/μL - eek! PCR'd with the slightly interior v2 UpF & DnR primers to get more construct 4x50 μL 1 μL acsA-up-F-2 1 μL acsA-down-R-2 1 μL Gel purified ligation product 1 μL dNTPs 1 μL Phusion 10 μL 5x Buffer HF 35 μL H2O 1:30 extension time, 72°C annealing temp. Run overnight.