User:Eric M. Walters/Notebook/Spring 2012/2012/05/21

Cyanobacterial acrylate production

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Producing the barcoded KO assembly I'll amplify the acsA-up and acsA-down regions with the half barcode sequence, then digest, and ligate together Amplifications Primer annealing is 68°C (NEB calc) These are a 1:10 dilution of the stocks 500bp amplification, I'll give it :15 seconds extension 10 uL HF buffer 1 uL dNTP 1 uL Phusion 1 uL fwd primer acsA-dnF-BCback-SphI or acsA-upF2 1 uL rev primer acsA-dnR2 or acsA-UpR-BCfront-SphI 1 uL 7002 gDNA 35 uL H20