User:Floriane Briere/Notebook/CHEM-496/2011/10/04

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Objective Today's experiment consists on performing a fluorescence spectrophotometry on [Ru(bpy)2-(5aminophen)](PF6)2 standard solution (whose concentration go from 0 to 100µM). Protocol By using the Ru-bpy stock solution (476µM) we are going to make 6 different standard solution. For each standard solution, we performed a fluorescence spectrum (excitation at 450nm; emission range from 500 to 700nm) Results Fluorescence intensity as a function of the wavelength Integration of the fluorescence intensity (corresponds to the area under each curve) We used excel to get these values Integrated value of the Fluorescence intensity as a function of the standard solution concentration