User:Helen L. Slucher/Notebook/CHEM 571/2013/10/02

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Objective

Monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol.

Procedure

  1. Chemiluminescence of luminol oxidation reaction initiated by stopped flow mixer
    1. Add HRP/Luminol stock solution to stopped flow mixer
    2. Add H2O2 stock solution to stopped flow mixer
    3. equilibrate mixer tubes with sample.
    4. Initiate Mixing
    5. Measure light produced as result of reaction, integrated over a specific time range
    6. Integrate area under the curve

Data (Luminescence Background)


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