User:Jarle Pahr/Electrophoresis

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Notes on gel electrophoresis

Plasmid DNA that has not been cut with a restriction endonuclease will migrate in 3 forms: supercoiled closed circular DNA, single-strand nicked open circle DNA, and sheared linear DNA.

Supercoiled plasmid DNA will migrate faster than linear forms.

The effect of loading dye may cause mismatch between sample DNA bands and the DNA ladder bands.



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