User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/09/10

iGEM UNAM-Genomics-Mexico

Main project page Previous entry      Next entry

September 10th, 2010 1. Replate transformation of BBa_I20260 Δ RBS into LB solid medium with Kan 30 because cells plated on September 9th, didn’t grow, 36 colonies were plated. 2. Make ligation of BBa_I20260 with the new RBS. Ligation methods (Total volume 20ul): DNA -> 7ul Buffer for T4 DNA ligase 5X -> 4ul (Final concentration 20%) T4 DNA ligase -> 1ul HPLC -> Complete total volume (20ul) Incubate overnight at 16ºC Mix well (vortex) buffer and reaction tubes, unfreeze buffer on ice.