09/30/15
- Outreach - Banana demo: transformations
Banana Scent demo
- For middle schoolers at Phoenix Country Day School
- 57 fifth grade students
Banana scent demo: transformations
- Banana: pTet-ATF1 (BBa_J45200) (Note: requires isoamyl alcohol to produce smell)
- Amp resistant
- Strain 1: BL21
- Strain 2: DH5α-turbo
- BL21 + BBa_J45200
- BL21 neg.
- DH5α-turbo + BBa_J45200
- DH5α-turbo neg.
- Transform with 0.5 uL DNA in 10 uL dH2O, or just water (neg)
- Use 50 μL chem. competent cells for each
- Plate on 100 μg/mL ampicillin
- Grow o/n at 37°C to get colonies
RESULTS
- ~100 colonies on DNA+plates
- 0 - 5 colonies on NEB and DH5α-turbo neg ctrls (respectively)
- Next: Pick colonies for liquid cultures and phenotype tests
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