User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/19

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01/19/10 ✓ Minipreps: KAH94, 95/JDS49 (6 each); KAH94, 95/JDS48 (6 each) ✓ Cultures: KAH139, 140, 141 (2 each) ✓ Cultures: KAH118/MV2 (12) Minipreps > Check with X/P digests Reagent Volume Expected: Empty: 3554, 2053, 1871 Fwd ins: 5500, 3039, 2053, 1871 Rev ins: 8039, 2053, 1871, 515 1-6. KAH94/JDS49 7-12. KAH95/JDS49 13-18. KAH94/JDS48 19-24. KAH95/JDS48 15 μL/lane; 1% agarose DNA(plasmid) 2.0 μL 10X buffer 1.5 XbaI 1.0 PstI 1.0 dH2O 9.5   15 μL --> 37°C/ ~15 min. --> Not sure about results. Check plasmids via PCR (RFP primers) tomorrow.